Larval survival at 48 hours post-fertilization for all cohorts. Percent survival 48 hours post-fertilization for buffered (B) and unbuffered (U) culture treatments for A) Cohort 1; B) Cohort 2; C) Cohort 3; and D) Cohort 4. Error bars indicate standard error. DOI: https://doi.org/10.1525/elementa.306.f3