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Table 1.
Materials for the anaerobic culturing of yogurt microorganisms.
Anaerobic chambers (both were successfully used)
  • OXO Pop Container, 5.5 L, airtight seal (OXO International, New York, NY)

  • Cracker Jar (glass), 3.8 L (Anchor Hocking Company, Lancaster, OH)

 
Petroleum jelly or Dow Corning High Vacuum Grease 
Hot Hands Hand Warmers (HeatMax Inc., Dalton, GA) 
Methylene Blue Indicator for Anaerobiosis (Brewer et al., 1966) 
  • 0.02% methylene blue indicator

 
  • 60% tris(hydroxymethyl)aminomethane

 
  • 4% dextrose

 
Greek yogurt, raspberry (Friendly Farms, Aldi, Batavia, IL) 
Note: Other types of yogurt may be suitable for this process although not all types of yogurt have been tested. It is recommended to check the ingredients of the yogurt to see what microorganisms are present and expected to be cultured from a particular yogurt. 
Phosphate-buffered saline (PBS), pH 7.4 (NaCl 8 g, KCl 0.2 g, Na2HPO4·7H2O 1.44 g, KH2PO4 0.24 g; distilled, deionized water, 1 L, bring to pH 7.4 with HCl). If the chemicals are not readily available, PBS can be ordered as a powder for preparation from Sigma-Aldrich (St. Louis, MO), P3813-1PAK or P3813-10PAK. If sterilizing the PBS via autoclaving is not possible, sterile PBS may be obtained from ABI-American BioInnovations, available from Amazon as Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL. Although we did not utilize it, sterile saline (0.9% NaCl) should also function to make dilutions of the yogurt to be plated. 
Brain Heart Infusion (BHI) Agar (Carolina Biological Supply Company, Burlington, NC, catalogue #781781) supplemented with 3 g/L agar for firmness. If the preparation of plates is not possible due to the lack of the appropriate facilities (e.g., an autoclave), prepared BHI plates, in packs of 10, may be obtained from Carolina Biological Supply Company, item #821282. BHI is a suitable medium as it contains the readily fermented substrate glucose, and although other media (Luria-Bertani broth, Trypticase Soy Agar, nutrient agar) have been suggested, we have not tested their suitability for the growth of the lactobacilli and for use with our system. 
Anaerobic chambers (both were successfully used)
  • OXO Pop Container, 5.5 L, airtight seal (OXO International, New York, NY)

  • Cracker Jar (glass), 3.8 L (Anchor Hocking Company, Lancaster, OH)

 
Petroleum jelly or Dow Corning High Vacuum Grease 
Hot Hands Hand Warmers (HeatMax Inc., Dalton, GA) 
Methylene Blue Indicator for Anaerobiosis (Brewer et al., 1966) 
  • 0.02% methylene blue indicator

 
  • 60% tris(hydroxymethyl)aminomethane

 
  • 4% dextrose

 
Greek yogurt, raspberry (Friendly Farms, Aldi, Batavia, IL) 
Note: Other types of yogurt may be suitable for this process although not all types of yogurt have been tested. It is recommended to check the ingredients of the yogurt to see what microorganisms are present and expected to be cultured from a particular yogurt. 
Phosphate-buffered saline (PBS), pH 7.4 (NaCl 8 g, KCl 0.2 g, Na2HPO4·7H2O 1.44 g, KH2PO4 0.24 g; distilled, deionized water, 1 L, bring to pH 7.4 with HCl). If the chemicals are not readily available, PBS can be ordered as a powder for preparation from Sigma-Aldrich (St. Louis, MO), P3813-1PAK or P3813-10PAK. If sterilizing the PBS via autoclaving is not possible, sterile PBS may be obtained from ABI-American BioInnovations, available from Amazon as Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL. Although we did not utilize it, sterile saline (0.9% NaCl) should also function to make dilutions of the yogurt to be plated. 
Brain Heart Infusion (BHI) Agar (Carolina Biological Supply Company, Burlington, NC, catalogue #781781) supplemented with 3 g/L agar for firmness. If the preparation of plates is not possible due to the lack of the appropriate facilities (e.g., an autoclave), prepared BHI plates, in packs of 10, may be obtained from Carolina Biological Supply Company, item #821282. BHI is a suitable medium as it contains the readily fermented substrate glucose, and although other media (Luria-Bertani broth, Trypticase Soy Agar, nutrient agar) have been suggested, we have not tested their suitability for the growth of the lactobacilli and for use with our system. 
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