Type of Analysis . | Description/Application . | Example . | Considerations . |
---|---|---|---|

Total abundance | Number of arthropods in a collected sample | 75 arthropods in a trap | Are students limited in how many arthropods they collect? |

Number of colonies cultured from an arthropod | 200 colonies on a plate | Colonies can grow together, or form lawns; students might estimate rather than count | |

Richness | Number of morphologically distinguishable arthropods | 6 species of crickets | Adults and immatures look different; similar-looking species |

Number of morphologically distinguishable colony types | 4 different types of colonies on a plate | Colonies might look similar; falsely interpreted Gram stains or cell shapes | |

Shannon index (H’) | Index of diversity of a sample; actual index can vary depending on the log base used | H’ = 1.5 (colonies on a plate) | Easily calculated with Excel formulas; higher H’ means more diversity |

Simpson index (D) | Index of diversity of a sample | D = 0.7 (colonies on a plate) | Easily calculated with Excel formulas; higher D means less diversity |

Jaccard index (Cj) | Index of similarity among a set of samples | Cj = 0.7 (two plates have most colony types in common) | Some equations for this can be intimidating! |

Fisher’s Exact test (P value) | Compares two outcomes between two groups (e.g., presence/absence of a microbe cultured from crickets vs. spiders) | P = 0.002 (microbe is significantly less likely to be present in one type of arthropod) | Only returns a P value; uses factorials; might be intimidating |

Type of Analysis . | Description/Application . | Example . | Considerations . |
---|---|---|---|

Total abundance | Number of arthropods in a collected sample | 75 arthropods in a trap | Are students limited in how many arthropods they collect? |

Number of colonies cultured from an arthropod | 200 colonies on a plate | Colonies can grow together, or form lawns; students might estimate rather than count | |

Richness | Number of morphologically distinguishable arthropods | 6 species of crickets | Adults and immatures look different; similar-looking species |

Number of morphologically distinguishable colony types | 4 different types of colonies on a plate | Colonies might look similar; falsely interpreted Gram stains or cell shapes | |

Shannon index (H’) | Index of diversity of a sample; actual index can vary depending on the log base used | H’ = 1.5 (colonies on a plate) | Easily calculated with Excel formulas; higher H’ means more diversity |

Simpson index (D) | Index of diversity of a sample | D = 0.7 (colonies on a plate) | Easily calculated with Excel formulas; higher D means less diversity |

Jaccard index (Cj) | Index of similarity among a set of samples | Cj = 0.7 (two plates have most colony types in common) | Some equations for this can be intimidating! |

Fisher’s Exact test (P value) | Compares two outcomes between two groups (e.g., presence/absence of a microbe cultured from crickets vs. spiders) | P = 0.002 (microbe is significantly less likely to be present in one type of arthropod) | Only returns a P value; uses factorials; might be intimidating |

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