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Table 1.

Overview of a laboratory exercise on bacterial growth.

Session 1 Discussion (20–30 minutes) of the activity’s importance; forming a hypothesis; and explanation of procedures and their purpose. Optical density (OD) readings taken for 140–180 minutes. Two samples taken at ~40 minutes and at 80–100 minutes for standard plating method. Serial dilutions prepared and plates inoculated. 
Session 2 Go over the growth-curve graphs that students prepared from OD data collected in session 1. Count colonies on plates prepared in session 1. Calculate colony-forming units/mL from data. Explanation of how doubling time is calculated from each method. Review the parts of a scientific paper and the rubric for grading. 
Session 1 Discussion (20–30 minutes) of the activity’s importance; forming a hypothesis; and explanation of procedures and their purpose. Optical density (OD) readings taken for 140–180 minutes. Two samples taken at ~40 minutes and at 80–100 minutes for standard plating method. Serial dilutions prepared and plates inoculated. 
Session 2 Go over the growth-curve graphs that students prepared from OD data collected in session 1. Count colonies on plates prepared in session 1. Calculate colony-forming units/mL from data. Explanation of how doubling time is calculated from each method. Review the parts of a scientific paper and the rubric for grading. 

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