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Table 2.

Molecular biology module schedule.

WeekActivityDescription & References
Genomic isolation DNA was isolated from bacteria using the DNeasy PowerSoil Kit (Qiagen). 
DNA fragmentation DNA was fragmented for library preparation. 
Library prep day 1 Fragmented DNA was end-prepped and A-tailed in preparation for adapter ligation, followed by cleanup using magnetic beads. 
Library prep day 2 Adapters were ligated and final PCR was performed, followed by cleanup using magnetic beads. 
Bioinformatics and poster presentation Once the sequencing results returned, students used BLAST (Altschul et al., 1990) and InterPro (Mitchell et al., 2018) to annotate function of antibiotic resistance genes. 
WeekActivityDescription & References
Genomic isolation DNA was isolated from bacteria using the DNeasy PowerSoil Kit (Qiagen). 
DNA fragmentation DNA was fragmented for library preparation. 
Library prep day 1 Fragmented DNA was end-prepped and A-tailed in preparation for adapter ligation, followed by cleanup using magnetic beads. 
Library prep day 2 Adapters were ligated and final PCR was performed, followed by cleanup using magnetic beads. 
Bioinformatics and poster presentation Once the sequencing results returned, students used BLAST (Altschul et al., 1990) and InterPro (Mitchell et al., 2018) to annotate function of antibiotic resistance genes. 
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