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Table 1.

Composition of the four test diets. DOI: https://doi.org/10.1525/elementa.2020.00170.t1

Ingredient (g/kg)Reference Diet (A)Spirulina Diet (B)Chlorella Diet (C)Schizochytrium Diet (D)
Fish meal 300 210 210 210 
Soybean meal 170 119 119 119 
Corn gluten meal 130 91 91 91 
Fish oil 100 70 70 70 
Wheat flour 280 196 196 196 
Vitamin/minerala 10 
Sipernat 50™.b 10 
Test ingredient 300 300 300 
Total 1,000 1,000 1,000 1,000 
Ingredient (g/kg)Reference Diet (A)Spirulina Diet (B)Chlorella Diet (C)Schizochytrium Diet (D)
Fish meal 300 210 210 210 
Soybean meal 170 119 119 119 
Corn gluten meal 130 91 91 91 
Fish oil 100 70 70 70 
Wheat flour 280 196 196 196 
Vitamin/minerala 10 
Sipernat 50™.b 10 
Test ingredient 300 300 300 
Total 1,000 1,000 1,000 1,000 

Test ingredient refers to the dried microalgae species being tested in each diet as indicated in the diet name. Proximate composition of each diet is reported in Sarker et al. (2016a).

aVitamin/mineral premix (mg kg–1 dry diet unless otherwise stated): vitamin A (as acetate), 7,500 IU kg–1 dry diet; vitamin D3 (as cholecalciferol), 6,000 IU kg–1 dry diet; vitamin E (as DL-a-tocopherylacetate), 150 IU kg–1 dry diet; vitamin K (as menadione Na-bisulphate), 3; vitamin B12 (as cyanocobalamin), 0.06; ascorbic acid (as ascorbyl polyphosphate), 150; D-biotin, 42; choline (as chloride), 3,000; folic acid, 3; niacin (as nicotinic acid), 30; pantothenic acid, 60; pyridoxine, 15; riboflavin, 18; thiamin, 3; NaCl, 6.15; ferrous sulphate, 0.13; copper sulphate, 0.06; manganese sulphate, 0.18; potassium iodide, 0.02; zinc sulphate, 0.3; carrier (wheat middling or starch).

bSipernat 50: Source of AIA comprises 98.50% SiO2 with an average particle size of 50 µm.

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