Although research and new technologies have introduced different ways of observing microorganisms, including scanning and electron microscopy, these methods are expensive and require equipment that is typically not found in a middle school classroom. The transmission-through-dye technique (TTD; Gregg et al., 2010 ), a new optical microscopy method that can be used with current basic light microscopes, relies on the fairly simple mechanism of filtered light passing through a dyed medium to produce an image that reflects cell thickness. With this technique, living microorganisms look bright red against a dark background, and movement can be seen easily among dead microorganisms and debris that show up black. Since the technique is low-cost and easy to implement, it addresses the needs of practitioners and is appropriate for a wide array of school contexts. We describe a three-week, hands-on, inquiry-based unit on TTD microscopy for middle and high school students.

Although research and new technologies have introduced different ways of observing microorganisms, including scanning and electron microscopy, these methods are expensive and require equipment that is typically not found in a middle school classroom. The transmission-through-dye technique (TTD; Gregg et al., 2010 ), a new optical microscopy method that can be used with current basic light microscopes, relies on the fairly simple mechanism of filtered light passing through a dyed medium to produce an image that reflects cell thickness. With this technique, living microorganisms look bright red against a dark background, and movement can be seen easily among dead microorganisms and debris that show up black. Since the technique is low-cost and easy to implement, it addresses the needs of practitioners and is appropriate for a wide array of school contexts. We describe a three-week, hands-on, inquiry-based unit on TTD microscopy for middle and high school students.

In an age-appropriate activity developed by a researcher-teacher team working together under the auspices of the American Chemical Science Coaches Program, middle school students at the Greensboro Montessori School (1) learned about the microbiological basis of fermentation, (2) learned about the chemical changes that take place during the fermentation of milk into yogurt, (3) applied this basic knowledge to designing and implementing experiments to test different conditions for culturing yogurt, (4) assessed the outcomes of different culturing conditions, and (5) developed a method for producing yogurt. This exercise includes hypothesis formulation, experimental design, and hypothesis testing and serves as an example of how empirically derived knowledge can be applied to the design of a food product.

Evolution explains both the unity and the diversity of all organisms, and developing students' ability to represent and communicate evolutionary relationships is an important component of a complete biology education. We present a series of student-centered, exploratory activities to help students develop their tree-thinking skills. In these activities, students use complementary phenotypic and molecular data to explore how to build phylogenetic trees and interpret the evolutionary relationships they represent. This learning module is designed to engage students in the process of science, provide them with active learning experiences using online bioinformatics tools, and foster their appreciation for the evolutionary connections across the tree of life.

With increasing focus on active learning in college classrooms, many institutions of higher education are redesigning introductory laboratory classes to provide more active-learning opportunities for students and to more authentically recreate the practices of scientists. These classes are primarily taught by graduate teaching assistants (GTAs), who often lack the pedagogical training necessary to plan for and support students' intellectual engagement in rich science tasks that require deep engagement in the practices of science and the core disciplinary ideas. We believe that graduate student discussion groups can provide an opportunity to encourage and equip GTAs with pedagogical knowledge and skills to select and use cognitively demanding instructional tasks. In this article, we describe our planning and facilitation of one such meeting with a group of GTAs about the relative cognitive demands of various laboratory activities. We propose that regularly scheduled meetings of discussion groups like this can help build learning communities among GTAs. We provide strategies to support GTAs' professional development and help them think critically about the tasks they use in their classes. In particular, we highlight the importance of the cognitive demands of tasks for engaging students in active and rigorous opportunities for science learning.

Students in a high school biology class were introduced to the case of “Marcus” (a pseudonym), a high school football player who collapsed on the football field during a game and was rushed to the emergency room with various symptoms. Throughout the two-week, project-based unit, students worked in cooperative groups to diagnose Marcus, learning about various inherited diseases and heat-related ailments that might impact young athletes. This unit integrates ultrasound technology into the classroom as a teaching and diagnostic technique and introduces students to health science careers. Student groups work to produce a final product that is presented to a public audience (e.g., parents, teachers, coaches) to increase their awareness of the science content underlying the causes of sudden collapses in young athletes. This learning experience ended with students individually writing a letter to Marcus's family explaining his diagnosis and the related biology concepts.

What is the role of wonder in a classroom? And how does a teacher activate this most human of emotions? This paper investigates the role wonder—and the subjects most closely associated with it, art and science—should play in our students’ school lives. The work connects my lived experiences as a high school teacher and college professor with the philosophy of John Dewey and contemporary literature on pedagogical practices. My findings suggest that it is a moral imperative to encourage wonder in our classrooms and to do so in an authentic way.

We investigated using Mathematica to create an interactive exercise that allows students to explore biological concepts. Exporting these active learning experiences to the Wolfram Computable Document Format (CDF) lets students enter data and modify parameters with only a browser plug-in and without needing to know how to use Mathematica. This exercise enhances student understanding by allowing them to focus on the biological concepts and visualize the data and calculations without the tedium of hand computation. In this article, we will highlight an interactive version of a biodiversity laboratory that explores Simpson's Diversity Index. Students are walked through sample calculations with a small data set, and then analyze a larger data set, which is presented as a set of interactive graphs and automatic calculations so that they can explore the data to answer questions. For our non-major audience, this laboratory leads to a more sophisticated discussion without getting stuck on the calculations. We will present the exercise and assessment results.

To create and implement meaningful tasks that go beyond the cognitive processes of understanding and that integrate all three dimensions of the Next Generation Science Standards (NGSS) is challenging for both educators and curriculum makers. This issue is compounded when considering a content-rich biology course such as anatomy and physiology that requires first familiarity and understanding before engagement in higher-order thinking. The use of crime scene investigations that encourages students to examine evidence even as they learn specific biology concepts can encourage meaning making about scientific practices and science content. This paper deconstructs the implementation of a crime scene investigation titled the “Jewel Heist,” created by the New York Hall of Science and implemented in twelfth-grade anatomy and physiology classes in a diverse urban high school in the northeastern United States. The NGSS, the Framework for K-12 Science Education , along with Bloom's taxonomy and Krathwohl's revisions, are implicated in this process.

The use of primary scientific inquiry and experimentation to develop students’ understanding of methodologies used by scientists and the nature of science is a key component of the Next-Generation Science Standards (NGSS). Introduction to inquiry-based experimentation also has been shown to improve students’ attitudes and interest in science. However, implementing scientific inquiry activities that include experimental design and data analysis in a classroom of middle or high school students can be daunting for teachers with limited experimental experience. Here, we present a four- to five-day, inquiry-based laboratory activity designed to teach students about the scientific process and excite them about scientific discovery while providing opportunities for interactions of both teachers and students with scientists in the field. Within this laboratory module, students make observations and develop their own research questions, then design, execute, analyze, and present the results of their hypothesis-driven experiments investigating the behavior of Caenorhabditis elegans , a relatively inexpensive and tractable model organism. Our experience running this module in a middle school biology classroom suggests students enjoyed the opportunity to investigate their own research questions, and post-course surveys indicated that students’ fear of biology decreased and their interest in biology-related careers increased following participation in the module.

This article describes a problem-based, cooperative learning activity, where students investigate the role of ethylene in flower senescence. The cooperative learning activity is contextualized in an authentic problem experienced in the cut flower industry: how can the shelf life of cut flowers be prolonged? We describe the procedure for conducting the experiment and show the affectiveness of contextualized science that includes indigenous knowledge—an approach that Gibbons calls “mode 2 knowledge production.” In addition we also give suggestions on how this type of problem-based, cooperative teaching-learning activity can be used in a school biology classroom.

To engage students in applying scientific process skills to real-world issues, we implemented a service-learning project model in our undergraduate introductory biology course for science majors. This model illustrates how we integrate inquiry inside and outside of the classroom through four steps: service, learning, classroom, and community. Out-of-class activities engaged students in serving the community ( Service step) while deepening their learning experience beyond what they would learn in a classroom ( Learning step). To connect the service-learning project with scientific process skills, students were asked to identify problems that our community partners were trying to solve, identify proposed solutions, and design ways to evaluate those solutions ( Classroom step). Additionally, students connected their service-learning topic with core concepts in Biology. After their service, students used metrics to analyze their impact. Students then synthesized the connection between their service, learning, and classroom projects by presenting their findings to the scientific and lay communities through a poster session ( Community step). Here we provide details of the model, recommendations, and examples for others to execute an inquiry-based service-learning project.

Traditionally, exploration of ecosystems in the context of undergraduate education has been restricted to connections within conventionally defined habitats (i.e., within a stream, within a forest). Further, instruction regarding the aquatic-terrestrial interface has emphasized directional inputs from land to water. However, a relatively new body of research has characterized reciprocal interactions and draws attention to fluxes from water to land, including the emergence of aquatic insects that serve as prey for terrestrial predators. We present a guide to an inquiry-based lesson for undergraduate biology that explores interactions and connections across aquatic and terrestrial habitat boundaries. The focus is on cross-habitat linkages within ecosystems, specifically addressing the question, What is the role of insect emergence in connecting the web of life linking aquatic and terrestrial habitats and organisms? Students (1) engage with a documentary film, (2) explore insect emergence and make observations of riparian insectivores, (3) explain the collected data, (4) elaborate on alternative study designs and a measure of ecosystem health, and (5) evaluate their new understanding. This lesson addresses core concepts and competencies for undergraduate biology education, as identified in the Vision and Change report.

Fish species are an important food resource all over the world, but the fishing practices of human beings are slowly driving many fish species to extinction. However, little is being done to communicate the problem of overfishing to the general public. In this three-part activity, students are introduced to the concepts of buoyancy and overfishing in an effort to provide a glimpse of how interesting fish are, and to raise awareness of overfishing. The students investigate buoyancy and gas compressibility by recreating a mysterious boat-sinking in the classroom, and by manipulating the buoyancy of artificial fish. By engaging in “fishing expeditions” with diminishing returns, the students learn why fish populations decline when we take too many of them. Throughout the activity, students have the opportunity to learn science as inquiry and the nature of science as presented in the National Science Education Standards.

The interdependence of living organisms and related ecology concepts are often difficult for students to grasp if they only study them from textbooks. To really understand how habitat fragmentation affects biodiversity, it is best to allow students to study it in the field. In the activities described here, I used inquiry as a basis for experiential learning. Focusing on two natural areas of unequal size, students investigated the areas to assess arthropod species richness and examine whether it was correlated with the size of the area. By establishing 10 daily observation periods and identifying arthropods in each session, students observed firsthand the relationship of species richness to biodiversity and that the size of the natural area was not significant. This translated to a greater understanding of biodiversity and its role in the relationships of living organisms in a local ecosystem. Students also gained valuable insight into how scientific studies are conducted.

Biology is often taught as disconnected facts, even though the subject itself provides a holistic approach to the study of life, particularly through the overarching frame of evolution. The Framework for K–12 Science Education and Next Generation Science Standards promote a coherent approach to science that uses a developmental approach to learning. This is consistent with the use of data, reflective strategies, and a research inquiry approach that encourages students to confront their own thinking and reasoning, and thus encourages the engagement of argumentation in the classroom. This article presents narratives and classroom scenarios that might provide insights into learning strategies, with implications for a more cohesive approach to learning both biology concepts and the practices of science.

First reported in March 2014, the Ebola virus disease (EVD) outbreak in West Africa has now claimed more lives than all other known EVD outbreaks combined, making it the deadliest occurrence of the disease since it was first discovered nearly 40 years ago. In hopes of turning the outbreak into something positive from an educational standpoint, a module was developed focusing on EVD, infectious disease, and epidemiology. The module engages students in a series of inquiry-based lessons, providing accurate and up-to-date information on the current outbreak of EVD in West Africa. The lessons also serve to correct popular misconceptions about the disease. The lessons include a jigsaw WebQuest using resources from the Centers for Disease Control and Prevention, a simulation based on fluid exchange to model the spread of an outbreak of infectious disease, and a “disease detective”–style mapping activity based on published data outlining the start of the current EVD outbreak in Guinea.

Population growth presents a unique opportunity to make the connection between mathematical and biological reasoning. The objective of this article is to introduce a method of teaching population growth that allows students to utilize mathematical reasoning to derive population growth models from authentic populations through active learning and firsthand experiences. To accomplish this, we designed a lab in which students grow and count populations of Drosophila over the course of 12 weeks, modifying abiotic and biotic limiting factors. Using the data, students derive exponential and logistic growth equations, through mathematical reasoning patterns that allow them to understand the purpose of these models, and hypothesize relationships between various factors and population growth. We gathered student attitudinal data and found that students perceived the lab as more effective, better at preparing them for lecture, and more engaging than the previous lab used. Through this active and inquiry-based method of teaching, students are more involved and engaged in both mathematical and biological reasoning processes.

The process of exploration and the methods that scientists use to conduct research are fundamental to science education. In this activity, authentic scientific practices are used to develop hypotheses to explain the natural world. Students observe grass shrimp in aquaria and construct an ethogram, which is a compilation of the observable behaviors an animal exhibits. They then conduct an experiment, just as real scientists would, to determine how changes in the environment alter shrimp behavior. This activity is designed for a fourth-grade science class and allows students to experience the excitement of observing a live organism while learning about scientific inquiry, and also reinforces quantification and graphing skills. “Do You See What I See” covers Next Generation Science Standards and addresses the science and engineering practices of engaging in argument from evidence.

The choice of the scientific method to be used depends on the question to be investigated, the type of study being performed, and the maturity of the particular subdiscipline. I review the scientific methods frequently used in biology since Darwin, the aspects of the nature of science relevant for teaching and learning about evolution, and some recent studies that tested the theory of evolution and some of its features. I also present some guidelines for teachers, within an inquiry-based instructional framework, to facilitate students’ understanding that hypothesis-driven and observation-driven studies are equally important and responsible for the advancement of scientific knowledge in the field of biology, both in the past and in the present.