...Kennedy Kam Ho Chan; Dickson Tik Shun Ho; David Siu Pan Lau Existing laboratory protocols for investigating the effects of factors affecting enzyme activity often require extensive hands-on manipulations and time. This can result in students either not getting the desired results or being...

... and chemicals readily available in high school laboratories. We show how the activity can help students understand how scientists can control the reaction rates of key enzymes involved in the digestion of dietary fat, to combat obesity. The activity also illustrates how instructional scaffolding can help...

... the measurement ( Figure 2e ). Figure 2. The process of measurement with the color sensor. Streptococcus salivarius ATCC 13419 was used as a source of urease. Catalytic reactions can be completed directly using microorganisms capable of producing enzymes. The microorganism releases the enzyme...

... by the enzyme conjugated to the antibody, produces a color change. The immunoblot is less expensive and faster to perform than plastic-plate-based assays. I have used this assay for over 10 years in undergraduate courses: in ecology to estimate disease incidence; in botany to explore immunological techniques...

.../reprintinfo.asp . 2012 Protein structure function model mutation enzyme 3-D The interrelationship of structure and function is a key theme of biology. One important example of this relationship involves how the three-dimensional shapes of proteins are related to the roles they play...