... samples from the National Park. They then ran a PCR with an rbcL primer pair to target the rubisco gene in plant chloroplasts. The PCR product was sequenced by a private company, Genewiz, and the DNA sequence was analyzed through DNA Subway. Twelve student groups, one or two students per group, isolated...

... how a pair of primers can be used to amplify a gene), whereas lab quizzes tended to focus on the main points of a particular test (e.g., List four of the components that need to be included in a PCR mix, prior to running PCR). Fish Sample Collection Students worked in teams of four throughout...

... replication, transcription, polymerase chain reaction (PCR), and random mutations so that students can examine these processes in detail. The set is inexpensive and easy to make, has been used successfully, and allows for modification to fit individual teachers' needs. © 2020 National Association of Biology...

... article content through the University of California Press's Reprints and Permissions web page, www.ucpress.edu/journals.php?p=reprints . 2019 DNA barcoding macroinvertebrates PCR polymerase chain reaction gel electrophorsis stream quality Figure 1. Sample images of common...

...′–OH group for the addition of new nucleotides) to PCR (primed DNA synthesis) and electrophoretic separation of DNA fragments. In addition, the lesson touches on some interesting aspects of the history of biology (e.g., Sanger vs. Maxam-Gilbert sequencing; why one survived and the other did...

... red and white onions bought from a local grocery store. A lack of red coloration in white onions is a result of one or more mutations in the color production pathway. This mutation can be seen by the use of polymerase chain reaction (PCR) followed by gel electrophoresis. An absence of an amplified PCR...

... Rights and Permissions Web site at http://www.ucpressjournals.com/reprintinfo.asp . 2015 Ecology genetics inquiry phenotype genotype PCR gel electrophoresis Middle school teachers face the challenge of covering a diverse array of topics in their biology or life sciences classes over...

... on the hellbender, reviews basic genetics, and exposes students to the scientific process that is used during PCR (polymerase chain reaction) and the analysis of microsatellites in order to calculate allele and genotype frequencies for understanding genetic variability. Once completing the lesson, students should...

.../reprintinfo.asp . 2013 PCR polymerase chain reaction CCR5 HIV resistance genotyping polymorphism secondary school Acquired immunodeficiency syndrome (AIDS) develops as a result of infection by human immunodeficiency virus (HIV), a virus that destroys various cells of our immune system...

...Angela R. Porta; Edward Enners The polymerase chain reaction (PCR) is a common technique used in high school and undergraduate science teaching. Students often do not fully comprehend the underlying principles of the technique and how optimization of the protocol affects the outcome and analysis...