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Keywords: PCR
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Journal Articles
The American Biology Teacher (2022) 84 (1): 40–44.
Published: 01 January 2022
... samples from the National Park. They then ran a PCR with an rbcL primer pair to target the rubisco gene in plant chloroplasts. The PCR product was sequenced by a private company, Genewiz, and the DNA sequence was analyzed through DNA Subway. Twelve student groups, one or two students per group, isolated...
Journal Articles
The American Biology Teacher (2020) 82 (3): 170–175.
Published: 01 March 2020
... in the “real lab world,” including a literature review, sample collection, mastering of key molecular biology techniques (DNA extraction, PCR, gel electrophoresis, PCR cleanup, DNA sequence analysis), conducting data analysis, and disseminating results by preparing a manuscript and a poster presentation. Our...
Journal Articles
The American Biology Teacher (2020) 82 (1): 49–51.
Published: 01 January 2020
... replication, transcription, polymerase chain reaction (PCR), and random mutations so that students can examine these processes in detail. The set is inexpensive and easy to make, has been used successfully, and allows for modification to fit individual teachers' needs. The set is made from inexpensive...
Includes: Multimedia, Supplementary data
Journal Articles
The American Biology Teacher (2019) 81 (3): 162–167.
Published: 01 March 2019
... article content through the University of California Press's Reprints and Permissions web page, www.ucpress.edu/journals.php?p=reprints . 2019 DNA barcoding macroinvertebrates PCR polymerase chain reaction gel electrophorsis stream quality In college introductory-level biology...
Journal Articles
The American Biology Teacher (2016) 78 (6): 516–522.
Published: 01 August 2016
... . 2016 DNA models Sanger sequencing DNA replication polymerase chain reaction PCR DNA structure and nucleic acid structure in general are highly abstract concepts. As such, they can present novice learners with difficulties as they try to understand the various aspects of DNA structure...
Journal Articles
The American Biology Teacher (2015) 77 (3): 211–214.
Published: 01 March 2015
... red and white onions bought from a local grocery store. A lack of red coloration in white onions is a result of one or more mutations in the color production pathway. This mutation can be seen by the use of polymerase chain reaction (PCR) followed by gel electrophoresis. An absence of an amplified PCR...
Journal Articles
The American Biology Teacher (2015) 77 (1): 50–54.
Published: 01 January 2015
.... Here, we show two alleles: one with nine repeats and one with 11 repeats. PCR using primers flanking the SSR region (arrows) produces fragments of different length from the alleles. Figure 2. Schematic of simple sequence repeat (SSR) regions within a genome. The boxes indicate a repeated sequence...
Journal Articles
The American Biology Teacher (2014) 76 (5): 338–344.
Published: 01 May 2014
... on the hellbender, reviews basic genetics, and exposes students to the scientific process that is used during PCR (polymerase chain reaction) and the analysis of microsatellites in order to calculate allele and genotype frequencies for understanding genetic variability. Once completing the lesson, students should...
Journal Articles
The American Biology Teacher (2013) 75 (9): 704–707.
Published: 01 November 2013
... the buccal cells A toothpick or a teaspoon as a scraping tool 0.2-mL PCR microtube (the type depends on the cycler used) 2–200 μL micropipette tip Students will share 2–20 μL (or equivalent) micropipette(s) PCR premix – prepare as described in the Procedure section while students...
Journal Articles
The American Biology Teacher (2012) 74 (4): 256–260.
Published: 01 April 2012
...Angela R. Porta; Edward Enners The polymerase chain reaction (PCR) is a common technique used in high school and undergraduate science teaching. Students often do not fully comprehend the underlying principles of the technique and how optimization of the protocol affects the outcome and analysis...