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Keywords: PCR
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Journal Articles
The American Biology Teacher (2020) 82 (3): 170–175.
Published: 01 March 2020
.../reprints-permissions . 2020 fish fraud mislabeling place-based learning DNA PCR gel electrophoresis Sanger sequencing It is increasingly important for undergraduate biology students to become proficient in laboratory techniques and to have an opportunity to engage in inquiry-based lab...
Journal Articles
The American Biology Teacher (2020) 82 (1): 49–51.
Published: 01 January 2020
... replication, transcription, polymerase chain reaction (PCR), and random mutations so that students can examine these processes in detail. The set is inexpensive and easy to make, has been used successfully, and allows for modification to fit individual teachers' needs. Figure 1. Components of the set: (1...
Journal Articles
The American Biology Teacher (2019) 81 (3): 162–167.
Published: 01 March 2019
...=reprints . 2019 DNA barcoding macroinvertebrates PCR polymerase chain reaction gel electrophorsis stream quality Figure 1. Sample images of common macroinvertebrates found in the streams of southwestern Pennsylvania, including the stonefly, identifiable by the two long antenna-like tails...
Journal Articles
The American Biology Teacher (2016) 78 (6): 516–522.
Published: 01 August 2016
... & M's. See Figure 2 for a sample outcome showing the template strand. Given this PCR primer, represent the set of different DNA fragment(s) that you could synthesize if a ddNTP (Peanut M & M) was incorporated into your products at low frequency. Photograph your set of products. See...
Journal Articles
The American Biology Teacher (2015) 77 (3): 211–214.
Published: 01 March 2015
... red and white onions bought from a local grocery store. A lack of red coloration in white onions is a result of one or more mutations in the color production pathway. This mutation can be seen by the use of polymerase chain reaction (PCR) followed by gel electrophoresis. An absence of an amplified PCR...
Journal Articles
The American Biology Teacher (2015) 77 (1): 50–54.
Published: 01 January 2015
... and one with 11 repeats. PCR using primers flanking the SSR region (arrows) produces fragments of different length from the alleles. Figure 2. Schematic of simple sequence repeat (SSR) regions within a genome. The boxes indicate a repeated sequence. Here, we show two alleles: one with nine repeats...
Journal Articles
The American Biology Teacher (2014) 76 (5): 338–344.
Published: 01 May 2014
... hellbender, reviews basic genetics, and exposes students to the scientific process that is used during PCR (polymerase chain reaction) and the analysis of microsatellites in order to calculate allele and genotype frequencies for understanding genetic variability. Once completing the lesson, students should...
Journal Articles
The American Biology Teacher (2013) 75 (9): 704–707.
Published: 01 November 2013
... with their own tissue absorbed students in this 2-hour practical. Table 1. PCR was run as follows. Steps 2a to 2c were repeated 35 times. Step Temperature (°C) Time 1. Initial denaturation 95 5 minutes 2a. Denaturation 95 20 seconds 2b. Annealing 60 20 seconds 2c...
Journal Articles
The American Biology Teacher (2012) 74 (4): 256–260.
Published: 01 April 2012
...Angela R. Porta; Edward Enners The polymerase chain reaction (PCR) is a common technique used in high school and undergraduate science teaching. Students often do not fully comprehend the underlying principles of the technique and how optimization of the protocol affects the outcome and analysis...