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tissue-specific-marker

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Journal Articles
The American Biology Teacher. 2020; 823175–177 doi: https://doi.org/10.1525/abt.2020.82.3.175
Published: 01 March 2020
... larger play area (recommended size: 18 × 18 inches). Red and blue beads can be purchased at any major craft store. Game tokens can be made by using air-dry clay and lollipop sticks. Blank dice can be purchased, and partial pressure values written on them with permanent marker ( Table 1 : custom dice...
Journal Articles
The American Biology Teacher. 2019; 817492–501 doi: https://doi.org/10.1525/abt.2019.81.7.492
Published: 01 September 2019
..., C. posadasii NR-4548, nonpathogenic strain (BEI-Resources) PCR marker (e.g., G3161, Fisher Scientific, cat. no. PR-G3161) Low Melting Agarose (Fisher Scientific, cat. no. AC159820100) 10x Tris-borate EDTA (TBE) buffer (Fisher Scientific, cat. no. NC0017762) Nucleic acid stain (SYBRSafe...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2019; 813162–167 doi: https://doi.org/10.1525/abt.2019.81.3.162
Published: 01 March 2019
... the first and last column empty for 3 μL of green 100 bp marking dye (Genemate Quanti Marker 100bp+; BioExpress, Kaysville, Utah). Run gels for 45 minutes on 100 V. Photograph gels under UV light and discard. The band size of each reaction can be used for identification of the organism classification...
Journal Articles
The American Biology Teacher. 2018; 804291–300 doi: https://doi.org/10.1525/abt.2018.80.4.291
Published: 01 April 2018
... Weighing boats Disposable Pasteur pipettes Labeling tape and marker Plastic wrap Watering can Lab notebook Growth space with fluorescent lights ( http://abrcoutreach.osu.edu/growing-arabidopsis-classroom ) Place 1.0 cubic feet of potting soil in a bucket or other large container...
Journal Articles
The American Biology Teacher. 2017; 799763–768 doi: https://doi.org/10.1525/abt.2017.79.9.763
Published: 01 November 2017
.... (Other nucleic acid stains such as SybrSafe or GelRed could also be used.) While the agarose was cooling, students prepared their samples for loading. We used a 100 bp DNA marker ladder (New England Biolabs, product # N3231S) so that students could identify the amplified 700 bp segment. This laboratory...
Journal Articles
The American Biology Teacher. 2017; 793208–223 doi: https://doi.org/10.1525/abt.2017.79.3.208
Published: 01 March 2017
..., www.ucpress.edu/journals.php?p=reprints . 2017 ß-catenin blastema Girardia sp. Dugesia dorotocephala odf2 Phagocata gracilis Phagocata morgani planarian maintenance regeneration RNA interference stem cells tissue-specific marker whole worm in situ hybridization Figure 1...
Journal Articles
The American Biology Teacher. 2017; 794282–287 doi: https://doi.org/10.1525/abt.2017.79.4.282
Published: 01 April 2017
... understand how genes regulate cell migration. Students learn to sort males from females, recognize fly genetic markers to identify wild type and mutant animals, hand-dissect ovaries, perform histochemical staining to reveal gene expression in this tissue, and visualize normal and aberrant cell migration...
Journal Articles
The American Biology Teacher. 2017; 79281–90 doi: https://doi.org/10.1525/abt.2017.79.2.81
Published: 01 February 2017
.../apcentral/Biology_VisualizingInformation.pdf Kong, Y. , Guo, B. Y. , Xie, C. X. , Fan, Q. X. , He, X. G. , Yang, R. B. , & Shao, J. ( 2009 ). The isolation via enrichment and characterization of 9 dinucleotide microsatellite markers in Pelteobagrus fulvidraco . Conservation Genetic...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2015; 778600–605 doi: https://doi.org/10.1525/abt.2015.77.8.6
Published: 01 October 2015
... storage bags Two scalpels with new blades (Carolina Biological Supply [CBS] no. 626642) Two pairs of 4.5 inch, stainless steel dissecting scissors (CBS no. 621810) Twenty to thirty dissecting pins (CBS no. 629130) Two small, padded dissecting trays (CBS no. 629005) One permanent marker...
Journal Articles
The American Biology Teacher. 2015; 772118–125 doi: https://doi.org/10.1525/abt.2015.77.2.7
Published: 01 February 2015
... the pituitary of the common marmoset ( Callithrix jacchus ). Journal of Molecular Endocrinology, 32 , 115–128. Osterholz, M., Vermeer, J., Walter, L. & Roos, C. (2008). A PCR-based marker to simply identify Saimiri sciureus and S. boliviensis boliviensis . American Journal of Primatology...
Journal Articles
The American Biology Teacher. 2014; 769589–594 doi: https://doi.org/10.1525/abt.2014.76.9.4
Published: 01 November 2014
...) $18.5 6 Plastic disposable pipette $1.25 1 Masking tape $2 6 Dice $3 6 Permanent markers $4.5 1 Tray or bucket to dispose of soil $5 6 Ruler $8 1–6 Balance NA 6 Weigh boats or paper towel $0 1 pack Microcentrifuge tubes $12 6 Forceps $12 1–6 Stereo...
Journal Articles
The American Biology Teacher. 2014; 76165 doi: https://doi.org/10.1525/abt.2014.76.1.14
Published: 01 January 2014
... organisms. Further changes follow from these gene changes and research using markers, stretches of DNA that travel with particular genes, has enabled scientists to link specific traits, such as the spines, to locations on specific chromosomes. Studies have shown that the presence of spines on sticklebacks...
Journal Articles
The American Biology Teacher. 2014; 76142–46 doi: https://doi.org/10.1525/abt.2014.76.1.9
Published: 01 January 2014
... and placed back in the cadaver storage closet when not in use. To protect the high-definition TV from rough handling, a piece of plexiglass covers the screen. Several lab groups use dry erase markers to label structures on the plexiglass and erase the marks when moving to the next station. The lab...
Journal Articles
The American Biology Teacher. 2013; 758559–565 doi: https://doi.org/10.1525/abt.2013.75.8.7
Published: 01 October 2013
... physical appearance of the tissue and for expression of diagnostic protein markers, which are both used to determine the tumor grade, candidacy for surgery, and likelihood of tumor recurrence. Tumor physical appearance is judged histologically: tissues are sectioned and placed on slides that are stained...
Journal Articles
The American Biology Teacher. 2012; 744224–230 doi: https://doi.org/10.1525/abt.2012.74.4.4
Published: 01 April 2012
... amplification. (B) Restriction mapping (“Rre I”) strategy for identification of A3243G. (C) Example of a student agarose gel following electrophoresis of samples. Lane 1: molecular weight marker; lane 2: normal DNA following restriction endonuclease (RE) treatment; lane 3: control A3243G DNA after RE treatment...
Journal Articles
The American Biology Teacher. 2012; 744213–223 doi: https://doi.org/10.1525/abt.2012.74.4.3
Published: 01 April 2012
... two repetitive elements, a long interspersed nucleotide element (LINE1) and a short interspersed nucleotide element (AluYb8), which are markers for global DNA methylation and, in addition, surveyed 1505 CpG sites in 807 promoters for gene-specific methylation changes. Exposed children showed lower...
Journal Articles
The American Biology Teacher. 2010; 728478–483 doi: https://doi.org/10.1525/abt.2010.72.8.3
Published: 01 October 2010
... microcentrifuge tubes •• Sharpie marker, extra-fine point •• Labeling tape •• Digital camera or colored pencils •• Dissecting microscope (optional) •• Computer with Internet access •• Freezer •• use an online taxonomic key to identify insects they have collected. •• extract genomic...
Journal Articles
The American Biology Teacher. 2009; 719525–530 doi: https://doi.org/10.2307/20565374
Published: 01 November 2009
..., R., Chakrabarty, T. & Manuelidis, L. (2008). A rapid accurate culture assay for infectivity in Transmissible Encephalopathies. Journal of Neurology, 14(5), 352-361. Lu, Z., Baker, C. & Manuelidis, L. (2004). New molecular markers of early and progres- sive CJD brain infection...
Journal Articles
The American Biology Teacher. 2009; 717406–410 doi: https://doi.org/10.2307/20565343
Published: 01 September 2009
... teaching evolutionary biology. The American Biology Teacher, 70, 30-33. O'Brien, S.J., Roelke, M.E., Marker, L., Newman, A., Winkler, CA., Meltzer, D., Colly, L., Evermann, J.F., Bush, M. & Wildt. D.E. (1985). Genetic basis for vulner- ability in the cheetah. Science, 227, 1428-1434...
Journal Articles
The American Biology Teacher. 2009; 714235–244 doi: https://doi.org/10.2307/27669417
Published: 01 April 2009
... observations much more difficult. Also, one Petri dish can be "divided" by using an indelible marker on the bottom of the plate to create two, three, or four sections for students to plate their individual pollen. THE AMERICAN BIOLOGY TEACHER MODEL PLANT 237 Camel hair brushes are frequently recommended for...