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southern-blot

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Journal Articles
The American Biology Teacher. 2012; 744270–271 doi: https://doi.org/10.1525/abt.2012.74.4.12
Published: 01 April 2012
...Derek M. Dean In this article, I describe an animated slideshow of Southern blotting that I have made freely available to other instructors. My hope is to provide a clear visualization of the logistics behind the technique so that instructors have a solid basis – as well as time freed up – to...
Images
Published: 01 April 2012
Figure 1. A visualization of the Southern/Northern blotting process for use in the classroom. (A) Standard agarose minigel that has had droplets of different food colorings laid on top of it, within the confines of a single lane. Each color is said to represent a nucleic acid of a specific size
Journal Articles
The American Biology Teacher. 2016; 788679–682 doi: https://doi.org/10.1525/abt.2016.78.8.679
Published: 01 October 2016
...) course for helping refine this experiment and contributing to the data presented in Figure 3 . Small, terrestrial, bufonid toads in the genus Anaxyrus (formerly Bufo ) are seasonally available from Carolina Biological Supply and Ward's Science (e.g., American Toads [ A. americanus ] and Southern...
Journal Articles
The American Biology Teacher. 2012; 749667–671 doi: https://doi.org/10.1525/abt.2012.74.9.14
Published: 01 November 2012
...:311-A, 5:OL-A Selective breeding.......................1:42-H Self-directed learning.......................6:387-A Sequencing.......................6:392-A Sexual selection.......................9:648-H Simulation.......................1:47-H Southern blot...
Journal Articles
The American Biology Teacher. 2012; 744232–236 doi: https://doi.org/10.1525/abt.2012.74.4.5
Published: 01 April 2012
.... What are the steps in the Southern blot procedure and restriction fragment length polymorphism? 7. What is one advantage and one disadvantage of staining a gel with ethidium bromide compared to methylene blue? 18. What is a degenerate restriction enzyme site? 8. Given the distance migrated and...
Journal Articles
The American Biology Teacher. 2007; 693163–168 doi: https://doi.org/10.2307/4452125
Published: 01 March 2007
...- satellites, a.k.a. variable number tandem repeats (VNTRs), consist of variants that are the result of 15-100 base pair (bp) repeated DNA fragments, and microsatellites are variants resulting from 2-7 bp repeated DNA fragments. VNTRs are commonly analyzed using the Southern blot procedure in order to detect...
Journal Articles
The American Biology Teacher. 2006; 68281–86 doi: https://doi.org/10.2307/4451936
Published: 01 February 2006
... itself and also to assist in gathering background information on cases from Internet sources. 2. Use the Case It! Sinmulation to test for the presence or absence of genetic mutations in hypothetical "family members" using RFLP analysis, Southern blotting, or dot blotting, and then save the graphic...
Journal Articles
The American Biology Teacher. 2003; 657548–549 doi: https://doi.org/10.2307/4451556
Published: 01 September 2003
...- ular biology with a minimum of expense. The techniques intro- duced include: Extracting DNA from Samples, DNA Fingerprint ing, Southern Blotting, RFLP (restriction fragment length polymorphism), and PCR (poly- merase chain reaction). It is rec- ommended to download this program only if you have a high...
Journal Articles
The American Biology Teacher. 2001; 635375 doi: https://doi.org/10.2307/4451131
Published: 01 May 2001
..., overhead masters, a web site list, and a glossary are provided. I was impressed with how a compli- cated procedure such as Southern blot- ting was explained in a clear and sim- ple manner on an overhead master. This is an exciting unit designed to engage students in the practical appli- cations of human...
Journal Articles
The American Biology Teacher. 2001; 638596–605 doi: https://doi.org/10.2307/4451191
Published: 01 October 2001
... techniques can be adapted for research projects by high school and undergrad- uate students (Vann et al. 1998). However, the expense associated with many of the reagents involved in RFLP analysis, and hazards-such as the use of radioactive materials for Southern blots-often prohibit carrying out true RFLP...
Journal Articles
The American Biology Teacher. 2001; 635363–367 doi: https://doi.org/10.2307/4451125
Published: 01 May 2001
... sequences of DNA that are repeated several times (i.e. VNTR). These regions are usually bounded by specific restriction sites and can be "cut" out and identified using the Southern blot procedure. Results of the Southern blots for each locus are given above. The samples are from all grandparents and from...
Journal Articles
The American Biology Teacher. 2002; 643212–218 doi: https://doi.org/10.2307/4451279
Published: 01 March 2002
... enzymes, direct visualization of discrete bands is not possible, but instead visualization requires a Southern blot transfer followed by hybridization and detection of a single-locus, or multi-locus, probe. The results obtained with the kit, therefore, are inconsistent with the method described in the...
Journal Articles
The American Biology Teacher. 2001; 635374–375 doi: https://doi.org/10.2307/4451130
Published: 01 May 2001
... activities, port- folio suggestions, guest speaker sug- gestions, overhead masters, a web site list, and a glossary are provided. I was impressed with how a compli- cated procedure such as Southern blot- ting was explained in a clear and sim- ple manner on an overhead master. This is an exciting unit...
Journal Articles
The American Biology Teacher. 2001; 635375–376 doi: https://doi.org/10.2307/4451132
Published: 01 May 2001
... activities, port- folio suggestions, guest speaker sug- gestions, overhead masters, a web site list, and a glossary are provided. I was impressed with how a compli- cated procedure such as Southern blot- ting was explained in a clear and sim- ple manner on an overhead master. This is an exciting unit...
Journal Articles
The American Biology Teacher. 2001; 636437–441 doi: https://doi.org/10.2307/4451151
Published: 01 August 2001
.... Depending on your objective for a particular class, this simula- tion can be used as a springboard for discussing VNTRs (variable number tandem repeats), Southern blotting and the application of radioac- tively labeled probes, PCR, and ethical questions related to DNA fingerprinting. Ellen Mayo and Anthony...
Journal Articles
The American Biology Teacher. 1997; 592125 doi: https://doi.org/10.2307/4450263
Published: 01 February 1997
... techniques are not given in the detail they deserve. Both Southern blots and polymerase chain reaction (PCR) are only very briefly presented. Oddly, neither technique is illustrated; although immunology and cloning are well-illustrated. A figure showing the details of primers binding to the region of DNA to...
Journal Articles
The American Biology Teacher. 2002; 6411–75 doi: https://doi.org/10.2307/4451225
Published: 01 January 2002
... Fuji Sensia film (ASA 100) for 1/60th of a second at f 4. Photographer William F. McComas is an Associate Professor and Director of the Center to Advance Science Education (www.science education.org) at the University of Southern California, Los Angeles, CA 90089-0031. C O N TE NT S Guest Editorial The...
Journal Articles
The American Biology Teacher. 2001; 639633–695 doi: https://doi.org/10.2307/4451203
Published: 01 November 2001
...-3409. Bringing Genomics into the Classroom Geneka Biotechnology introduces a complete line of educational kits designed to highlight such techniques as the Gelshift/EMSA, the Northern blot and the Western blot. Laboratory techniques that once seemed quite complex are simplified. Students learn to...
Journal Articles
The American Biology Teacher. 2000; 62178 doi: https://doi.org/10.2307/4450834
Published: 01 January 2000
... has done a remark- able job of bringing together a great variety of insights, case histories and scientific information. His skill in explaining complex techniques and terminology is considerable, and the illustrations and descriptions of RFLP analysis, Southern blot analysis, and molecular cloning...
Journal Articles
The American Biology Teacher. 2001; 637468–533 doi: https://doi.org/10.2307/4451164
Published: 01 September 2001
... School, NY St. Benedict High School, IL St. Bernard High School, CA St. Brendan High School, FL St. Francis School, GA St. John Central High School, OH St. Mary's Girls Academy High School, CO St. Mary's High School, KS Scotus Central Catholic School, NE Southern Cayuga High School, NY Stow High School...