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situ-hybridization

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Journal Articles
The American Biology Teacher. 2006; 684227–231 doi: https://doi.org/10.2307/4451971
Published: 01 April 2006
...William P. Baker; Carleton Buck Jones Copyright National Association of Biology Teachers References Beatty, B. (2002). FISH (Fluorescence In Situ Hybridization). New York: Oxford University Press. Beatty FISH (Fluorescence In Situ Hybridization) 2002 Gehlehrter, T., Ginsburg...
Journal Articles
The American Biology Teacher. 2017; 793208–223 doi: https://doi.org/10.1525/abt.2017.79.3.208
Published: 01 March 2017
... complex whole worm in situ hybridizations, and RNA interference for gene knock-down experiments are described for each of the four species. Cumulatively, the suggested methods and experiments will facilitate the exploration of animal regeneration biology and promote curiosity-driven, hands-on application...
Journal Articles
The American Biology Teacher. 2010; 724245–251 doi: https://doi.org/10.1525/abt.2010.72.4.9
Published: 01 April 2010
...Jamie L. Jensen I present a learning cycle that explores different biotechnologies using the process of in situ hybridization as a platform. Students are presented with a cyclopic lamb and must use biotechnology to discover the mechanism behind the deformity. Through this activity, students learn...
Images
All panels depict the non-commercially available species  Schmidtea mediter...
Published: 01 March 2017
Figure 3. All panels depict the non-commercially available species Schmidtea mediterranea . (A) Colorimetric whole mount in situ hybridization (WISH) detects piwi-1 gene expression and illustrates planarian neoblast (stem cell) distribution. (B) Regeneration time course after head Figure 3. All panels depict the non-commercially available species Schmidtea mediterranea . (A) Colorimetric whole mount in situ hybridization (WISH) detects piwi-1 gene expression and illustrates planarian neoblast (stem cell) distribution. (B) Regeneration time course after head More
Journal Articles
The American Biology Teacher. 2018; 808561–569 doi: https://doi.org/10.1525/abt.2018.80.8.561
Published: 01 October 2018
... increased Ultrabithorax ( Ubx ) expression during insect development? If an Ubx RNAi experiment was conducted in each of the species, which of the hind-leg segments would be affected? Looking at isolated in situ hybridization images from an Orthopteran embryo, which is the fore-leg and which is...
Journal Articles
The American Biology Teacher. 2017; 793176–183 doi: https://doi.org/10.1525/abt.2017.79.3.176
Published: 01 March 2017
.... References Bichlmeier, C. , Wimmer, F. , Heining, S. M. , & Navab, N. ( 2007 , November ). Contextual anatomic mimesis hybrid in-situ visualization method for improving multi-sensory depth perception in medical augmented reality . In Mixed and Augmented Reality, 2007. ISMAR 2007. 6th IEEE and...
Journal Articles
The American Biology Teacher. 2015; 77163–67 doi: https://doi.org/10.1525/abt.2015.77.1.9
Published: 01 January 2015
... single-nucleotide polymorphisms, fluorescent in situ hybridization (FISH) or chromosomal microarray analysis can be performed. FISH analysis involves identifying specific sequences on chromosomes using a smaller piece of fluorescently labeled DNA that base-pairs with the DNA within the chromosomes...
Journal Articles
The American Biology Teacher. 2013; 759692–697 doi: https://doi.org/10.1525/abt.2013.75.9.11
Published: 01 November 2013
... Human Karyotyping worksheet to support your answer. References Baker, W.P. & Jones, C.B. (2006). FISH-ing for genes: modeling fluorescence in situ hybridization. American Biology Teacher , 68 , 227–231. Basu, S.J. & Calabrese Barton, A. (2007). Developing a sustained interest in...
Journal Articles
The American Biology Teacher. 2008; 705284–287 doi: https://doi.org/10.2307/30163274
Published: 01 May 2008
... fluorescent in situ hybridization (FISH). With PCR, a small section of the DNA is repeatedly copied (called amplification). This creates bil- lions of copies of a specific section of DNA that can be used for further testing. For PGD, genes associated with different diseases are amplified. PCR is performed by...
Journal Articles
The American Biology Teacher. 2006; 689559–562 doi: https://doi.org/10.2307/4452055
Published: 01 November 2006
..., 3:170R, 5:35online, 5:275A, 9:570R Evolution Lecture/Discussion 7:438C Experimental Design 6:3541 Fecal Coliform 5:296H Fermentation 4:25online Field Guide 6:362H Field Trips 6:61online, 6:67online F.I.S.H . 4:2271 Fluorescence i situ Hybridization 4:2271 Forensic Entomology 7:4021 Forensic S ience...
Journal Articles
The American Biology Teacher. 2009; 71295–98 doi: https://doi.org/10.2307/27669379
Published: 01 February 2009
..., while also fostering an appreciation for how the theory aids us in addressing real-world issues. Framing this unit in a relevant context meant exploring a situ ation to which students were currently exposed. The previous year, in an Indiana suburban high school at which my teacher-collabora tor taught...
Journal Articles
The American Biology Teacher. 2006; 685307–314 doi: https://doi.org/10.2307/4451999
Published: 01 May 2006
... specimen or that records the results of procedures used to analyze the macromolec- ular constituents of the specimen (such as the detec- tion of proteins using immunohistochemical methods or nucleic acids using in situ hybridization tech- niques). In theory, the process for acquiring a pho- tomicrograph is...
Journal Articles
The American Biology Teacher. 2008; 703153–157 doi: https://doi.org/10.2307/30163228
Published: 01 March 2008
..., mocking thrush, finches and numerous plants. These species having the same general habits, occupying analogous situ- ations and obviously fitting the same place on the natural economy of this archipelago that strikes me with wonder. -Darwin, 1845, p. 409 Darwin's use of the term "natural economy," another...
Journal Articles
The American Biology Teacher. 2006; 684208–252 doi: https://doi.org/10.2307/4451963
Published: 01 April 2006
.... Abramson 221 FISH-ing for Genes: Modeling Fluorescence in situ Hybridization Teachers with limited resources can effectively modelfluorescence in situ hybridization with readily available resources. Learn to FISH with results! William P. Baker Carleton Buck Jones 227 How-To-Do-Its Developing Inquiry-Based...
Journal Articles
The American Biology Teacher. 2007; 699565–568 doi: https://doi.org/10.2307/4452227
Published: 01 November 2007
... Pare and his peers can still potently problematize our notions of male and female (Sacred Bovines, Aug., 2006). As a modern example, situs iniversus, the lateral reversal of internal organs (McManus, 2002), might also disturb our sense of the natural order. Non-human examples, such as albino gorillas...
Journal Articles
The American Biology Teacher. 2006; 689530–535 doi: https://doi.org/10.2307/4452060
Published: 01 November 2006
... resistant strain may perform bet- ter than the susceptible strain in some situ- ations (Tables 4 and 5). Data Set 3 comes from an experiment performed under low light conditions (two-bulb fluorescent light bank). In this situation resistant plants were taller than susceptible plants in two com- parisons, R...
Journal Articles
The American Biology Teacher. 1994; 567445 doi: https://doi.org/10.2307/4449881
Published: 01 October 1994
...- cedures are Southern and Northern blotting techniques, electrophoresis, and a new method, FISH (fluorescent in situ hybridization), which has proven to be sensitive and precise in determining DNA sequences. Chapters 4 through 6 discuss clon- ing techniques and future prospects. Again the authors...
Journal Articles
The American Biology Teacher. 1994; 567445 doi: https://doi.org/10.2307/4449882
Published: 01 October 1994
... crossing-over and sequence analysis. Mentioned as pro- cedures are Southern and Northern blotting techniques, electrophoresis, and a new method, FISH (fluorescent in situ hybridization), which has proven to be sensitive and precise in determining DNA sequences. Chapters 4 through 6 discuss clon- ing...
Journal Articles
The American Biology Teacher. 1994; 567444–445 doi: https://doi.org/10.2307/4449880
Published: 01 October 1994
... Chapters 2 and 3, the discussion focuses on gene analysis techniques, with a portion of the dis- cussion examining crossing-over and sequence analysis. Mentioned as pro- cedures are Southern and Northern blotting techniques, electrophoresis, and a new method, FISH (fluorescent in situ hybridization), which...
Journal Articles
The American Biology Teacher. 1984; 467384–387 doi: https://doi.org/10.2307/4447876
Published: 01 October 1984
... then hybridized with the isotope labeled DNA probe. Radioactive hybridized DNA is visualized by plac- ing the nitrocellulose membrane on X-ray film. Blackened silver grains will appear on the film cor- responding to the position of the radioactive hybridized DNA. In situ hybridization, the...