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mrna-sequencing

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Example <b>mRNA</b> <b>sequences</b>.   Figure 4. Example <b>mRNA</b> <b>sequences</b>.
Published: 01 April 2012
Figure 4. Example mRNA sequences. Figure 4. Example mRNA sequences. Figure 4. Example mRNA sequences. Figure 4. Example mRNA sequences. More
Journal Articles
The American Biology Teacher. 2011; 737418 doi: https://doi.org/10.1525/abt.2011.73.7.9
Published: 01 September 2011
...Evan Lampert I explain a classroom activity to model translation of RNA into proteins. Students are given worksheets with short mRNA sequences and a genetic code coding phrases instead of amino acids. Students use the code to write a sentence from their mRNA sequences; a "Chuck Norris fact" is...
Journal Articles
The American Biology Teacher. 2012; 744262–265 doi: https://doi.org/10.1525/abt.2012.74.4.10
Published: 01 April 2012
...Figure 4. Example mRNA sequences. Figure 4. Example mRNA sequences. ...
Images
Example <b>mRNA</b> to amino acid <b>sequence</b> algorithm.   Figure 7. Example <b>mRNA</b> to ...
Published: 01 January 2018
Figure 7. Example mRNA to amino acid sequence algorithm. Figure 7. Example mRNA to amino acid sequence algorithm. Figure 7. Example mRNA to amino acid sequence algorithm. Figure 7. Example mRNA to amino acid sequence algorithm. More
Journal Articles
The American Biology Teacher. 2016; 783254–256 doi: https://doi.org/10.1525/abt.2016.78.3.254
Published: 01 March 2016
... constitute them. The exercises presented here focus on DNA replication and the translation of mRNA into amino acid sequences, multistep processes that require specific series of events. The exercises help students appreciate the spatial and temporal frameworks of these essential cellular functions. They can...
Journal Articles
The American Biology Teacher. 2019; 813202–209 doi: https://doi.org/10.1525/abt.2019.81.3.202
Published: 01 March 2019
... dogma of molecular biology (DNA → RNA → protein) and eventually genetics, students first need to understand the relationship between DNA, mRNA, and proteins, and subsequently that between protein function and disease. It is critical they understand why the genetic code uses three consecutive nucleotides...
Journal Articles
The American Biology Teacher. 2018; 809642–648 doi: https://doi.org/10.1525/abt.2018.80.9.642
Published: 01 November 2018
... genes appeared to be straightforward templates for mRNA molecules, with these appearing to be straightforward templates for the amino-acid sequence of proteins. However, the nature of the processing that primary mRNA undergoes on the way to becoming functional mRNA insures that there is no one-to-one...
Journal Articles
The American Biology Teacher. 2018; 808561–569 doi: https://doi.org/10.1525/abt.2018.80.8.561
Published: 01 October 2018
... message into a protein occurs. In situ hybridization is intended to detect the presence of specific cytoplasmic mRNA in tissues/tissue sections of interest. This is accomplished by using a specifically designed piece of single-stranded RNA, or “probe,” which serves as a reverse complement to the mRNA...
Journal Articles
The American Biology Teacher. 2018; 804278–284 doi: https://doi.org/10.1525/abt.2018.80.4.278
Published: 01 April 2018
.... In introductory biology courses, students learn to translate the nucleotide sequence of a protein-coding gene into an amino acid sequence using the mRNA codon chart. In ExPASY, students copy and paste their edited sequences into a translation window, which executes the same process in a much shorter...
Journal Articles
The American Biology Teacher. 2018; 80121–28 doi: https://doi.org/10.1525/abt.2018.80.1.21
Published: 01 January 2018
...Figure 7. Example mRNA to amino acid sequence algorithm. Figure 7. Example mRNA to amino acid sequence algorithm. ...
Journal Articles
The American Biology Teacher. 2018; 8012–5 doi: https://doi.org/10.1525/abt.2018.80.1.2
Published: 01 January 2018
... Individual and group quizzes with IFAT cards Diagramming transcription and translation as groups Think-pair-share question: What are at least three differences and similarities between transcription and translation? CF problem sets: Diagram the CFTR gene, most common mRNA transcript, and...
Journal Articles
The American Biology Teacher. 2017; 794257–271 doi: https://doi.org/10.1525/abt.2017.79.4.257
Published: 01 April 2017
...., disulfide bridge-forming cysteines; salt bridge-forming charged amino acids: K ⊕ R ⊕ —E ⊖ D ⊖ ). ⑤ “T3 Reverse”: Give students protein structural models and have them reverse-engineer binary, amino acid, mRNA, and DNA sequences. ⑥ “Designer Proteins”: De novo protein design is an exciting area...
Journal Articles
The American Biology Teacher. 2017; 793208–223 doi: https://doi.org/10.1525/abt.2017.79.3.208
Published: 01 March 2017
... suggested sequence or sequences? D. Section: “RNAi: Disruption of Gene Function” Goals: Acquisition of basic molecular biology knowledge and abilities; acquisition of knowledge of both the importance of mRNAs for normal cell functioning and the potential problems caused by missing, truncated or...
Journal Articles
The American Biology Teacher. 2016; 786482–491 doi: https://doi.org/10.1525/abt.2016.78.6.482
Published: 01 August 2016
...” Chloroplasts intercept light and manufactures sugar Cell wall provides structure The cytoskeleton (or microtubules) provide structural support and facilitate the transport of molecules Kinesin is a transport motor protein. mRNA is made from DNA Protein is made from mRNA Vacuole is for water...
Journal Articles
The American Biology Teacher. 2015; 773185–190 doi: https://doi.org/10.1525/abt.2015.77.3.6
Published: 01 March 2015
... The mRNA with its poly-A tail and cap is laid on the small ribosomal subunit ( Figure 2 ). The student acting as the initiator tRNA with the corresponding anticodon (worn around the waist) “attaches” to the start codon of the mRNA by sitting on the 5´AUG3´ sequence of the mRNA. The Velcro of the...
Journal Articles
The American Biology Teacher. 2016; 782127–135 doi: https://doi.org/10.1525/abt.2016.78.2.127
Published: 01 February 2016
.... Describe the role of mRNA, rRNA (ribosomes), and tRNA during the process of translation. Analyze how mutations or changes in the sequence of DNA can lead to changes in the proteins produced. References Groenke, S.L. & Puckett, R. ( 2006 ). Becoming environmentally literate citizens...
Journal Articles
The American Biology Teacher. 2016; 782155–162 doi: https://doi.org/10.1525/abt.2016.78.2.155
Published: 01 February 2016
.... Figure 4. Results of fossil phenotype analysis. Figure 7. Model of genetic regulation by “gene switches.” Figure 7. Model of genetic regulation by “gene switches.” Figure 8. Pitx1 mRNA expression in marine and freshwater sticklebacks (adapted from Shapiro et al., 2004 ). Figure 8...
Journal Articles
The American Biology Teacher. 2015; 776445–451 doi: https://doi.org/10.1525/abt.2015.77.6.445
Published: 01 August 2015
... on patient samples to determine percent activity of GAA and use genetic analysis to confirm the result. Students identify mutations in the patient DNA, transcribe the sequence into mRNA, and translate into amino acids. Protein models are created and compared to a normal GAA protein model as students...
Journal Articles
The American Biology Teacher. 2015; 772118–125 doi: https://doi.org/10.1525/abt.2015.77.2.7
Published: 01 February 2015
.... (2005). Short retroposons in eukaryotic genomes. International Review of Cytology, 247 , 165–221. Müller, T., Simoni, M., Pekel, E., Luetjens, C.M., Chandolia, R., Amato, F. & others (2004). Chorionic gonadotropin beta subunit mRNA but not luteinizing hormone beta subunit mRNA is expressed in...
Journal Articles
The American Biology Teacher. 2015; 773211–214 doi: https://doi.org/10.1525/abt.2015.77.3.10
Published: 01 March 2015
... varieties of yellow, golden, and pink onions from the grocery to determine which genes might be defective. For more advanced inquiry, students could use RNA extraction and reverse transcriptase (RT) with PCR to evaluate gene expression (mRNA) in various organs of the plant (i.e., leaves, roots, and bulbs...