Search Results for mini-centrifuge
1-20 of 27 Search Results for
The American Biology Teacher. 2018; 806451–456 doi: https://doi.org/10.1525/abt.2018.80.6.451
Published: 01 August 2018
... a result, we describe how to construct a “homemade” single-speed mini-centrifuge using inexpensive parts easily acquired online or at hardware or other stores (e.g., a 4.7-quart plastic bowl and lid, standard computer cooling fan, AC/DC adapter, electrical wire, etc.). We further used this design...
Published: 01 August 2018
Figure 6. The relative frequency of Tetrahymena thermophila cells in the middle of the supernatant as a function of centrifugation time (0, 10, 20, 40, or 80 seconds) in a Gilson GmC Lab mini-centrifuge or the OPN Minifuge. Figure 6. The relative frequency of Tetrahymena thermophila cells in Figure 6. The relative frequency of Tetrahymena thermophila cells in the middle of the supernatant as a function of centrifugation time (0, 10, 20, 40, or 80 seconds) in a Gilson GmC Lab mini-centrifuge or the OPN Minifuge. Figure 6. The relative frequency of Tetrahymena thermophila cells in More
The American Biology Teacher. 2018; 809697–701 doi: https://doi.org/10.1525/abt.2018.80.9.697
Published: 01 November 2018
... Biomagnification to Illustrate Trophic Pyramids in the Middle School Classroom............................5.385-TT An Inexpensive, Open-Source Mini-Centrifuge............................6.451-TT Using Open-Source Data in Correlative Species Distribution Modeling of Marine Species...
The American Biology Teacher. 2018; 804285–289 doi: https://doi.org/10.1525/abt.2018.80.4.285
Published: 01 April 2018
... the commercially available QIAGEN DNA mini kit (#51304, QIAGEN, Valencia, CA) used in many research laboratories. After mixing the samples by inverting the 50 mL centrifuge tubes, students pull out a 200 µL subsample, which is then processed using the extraction procedure provided in the kit. Even...
Includes: Supplementary data
The American Biology Teacher. 2017; 793191–197 doi: https://doi.org/10.1525/abt.2017.79.3.191
Published: 01 March 2017
... increasingly comfortable with the procedures. We also informally ask them questions about their project throughout the program. At the end of the five weeks students are required to give a brief talk in which they explain their project and results, and to present a poster at a mini-symposium. We have used...
The American Biology Teacher. 2016; 788669–675 doi: https://doi.org/10.1525/abt.2016.78.8.669
Published: 01 October 2016
... the yeast mini-respirometer Q.2 Explain the purpose of (a) using boiled water (b) using water cooled to room temperature rather than boiling water (c) adding the paraffin oil to the setup. Q.3 Discuss with your peers to plan for the observations you will make in this practical...
The American Biology Teacher. 2012; 744237–243 doi: https://doi.org/10.1525/abt.2012.74.4.6
Published: 01 April 2012
... Mini/microcentrifuge 8,000 or 15,000 × g SDS-PAGE chamber with appropriate power supply Precast gels Use Tris-HCl 12% gel that meet chamber specifications Micropipettes of varying sizes with appropriate tips Microcentrifuge tubes Hot water bath (95°C) Although there is a...
The American Biology Teacher. 2009; 716363–366 doi: https://doi.org/10.2307/20565332
Published: 01 August 2009
... DNA Mini-Prep from Bacteria 1. Grow bacterial culture in 2 ml of medium with appropriate antibiotic over night. 2. Add 1.5 ml culture to microcentrifuge tube. 3. Spin in centrifuge for one minute at maximum speed. 4. Pour off media. 5. Resuspend pellet in 100 p1 Solution I. 6. Add 200 p1 Solution II...
The American Biology Teacher. 2008; 709537–545 doi: https://doi.org/10.2307/27669339
Published: 01 November 2008
...://www.biointeractive.org. Jenkins, K.P. & Bielec, B. (2006). Running DNA mini-gels in 20 minutes or less using sodium boric acid buffer. The American Biology Teacher, 68(9), 544-546. Kass, D.H. (2007) Simple and rapid generation of complex DNA profiles for the undergraduate laboratory. The American Biology...
The American Biology Teacher. 2007; 695280–286 doi: https://doi.org/10.2307/4452157
Published: 01 May 2007
... profiling unit as a one-week sum- mer mini-course, however the activities can be arranged to complement a genetics or biotechnology unit within a regu- lar semester. Students genotype themselves first to learn the technique, then recover DNA from saliva left at a fictitious crime scene and from suspects for...
The American Biology Teacher. 2004; 664291–296 doi: https://doi.org/10.2307/4451672
Published: 01 April 2004
... Preparation To make practice gel plates, agarose, IX TBE (Tris- Boric Acid-EDTA) buffer, 100 x 15 mm petri dishes, and mini electrophoresis gel combs are needed. To prepare 500 ml 1% agarose, heat 500 ml IX TBE buffer and 5 g agarose in a 1 L beaker or flask on a hot plate (or in a microwave oven) and bring...
The American Biology Teacher. 2004; 663192–198 doi: https://doi.org/10.2307/4451653
Published: 01 March 2004
... easily. However, at the end, they feel like they have carried out a "real" experiment. We have our stu- dents work in groups to promote bonding within the class, to reduce the cost of the experiment, and to mini- mize the amount of equipment required. We have observed that some groups work together much...
The American Biology Teacher. 2002; 642122–129 doi: https://doi.org/10.2307/4451255
Published: 01 February 2002
... cells (RBCs) were also sedimented in a MSE GT-2 clinical centrifuge (often available in anato- my/physiology or hematology labs) at 1200 rpm (-250 X g) for 5 minutes or in a VWR personal Mini Centrifuge (VWR Scientific Products catalog #20668- 212, $290) at 6000 rpm (2000 X g) for 2 minutes. Likewise...
The American Biology Teacher. 2003; 658615–618 doi: https://doi.org/10.2307/4451572
Published: 01 October 2003
... millions of mini-bioreactors, each using a series of enzyme-catalyzed pathways to generate electrons while they metabolized the glucose substrate in the nutrient broth. The result was a measurable increase in the volt- age through the fuel cell. Experiments To Explore with the Biological Fuel Cell We have...
The American Biology Teacher. 2002; 646433–442 doi: https://doi.org/10.2307/4451335
Published: 01 August 2002
...% isopropanol to each minicol- umn and reapply the vacuum to draw the solu- tion through the minicolumn. This step washes the column. 14. Remove the syringe barrel and transfer the mini- column to a 1.5 mL microcentrifuge tube. Centrifuge the minicolumn at 10,000 x g in a microcentrifuge for 2 minutes to remove...
The American Biology Teacher. 2000; 624288–291 doi: https://doi.org/10.2307/4450898
Published: 01 April 2000
... CaCl2 (3.62 g CaCl2, 5 ml, 1 M Tris, pH 8, per 500 ml)] was aliquoted to sterile centrifuge tubes and placed on ice. 250 LI of the calcium chloride solution is required per student. 3. Plasmid isolation. Commercial mini-prep kits were purchased from either Promega Biotech, Madison, WI (Wizard Plus spin...
The American Biology Teacher. 1998; 604276–280 doi: https://doi.org/10.2307/4450471
Published: 01 April 1998
... complex. The fluorescence is measured using a Hoefer 100 Mini- fluorometer (Hoefer Scientific Instru- ments, San Francisco, CA, USA) cali- brated with purified Escherichia coli DNA (Trevors 1992). DNA concentra- tions in the ng range can be quantified with this method. A sample as small as 2 ul can be...
The American Biology Teacher. 1998; 606448–452 doi: https://doi.org/10.2307/4450519
Published: 01 June 1998
... base, 27.5 g of boric acid, 20 ml of a 0.5 M EDTA solution (pH 8), and deionized water to a final volume of 1 liter (Sambrook et al. 1989). Pre-mixed TBE solutions are also available commercially. 30 ml, 0.6% gels were cast and run in a BlA Owl Easy-cast mini-gel system with a 7 cm x 8 cm gel with and...
The American Biology Teacher. 1997; 599580–583 doi: https://doi.org/10.2307/4450386
Published: 01 November 1997
... test aqueous samples obtained from polluted sites, such as ponds or streams near gasoline stations or landfills. These studies can be further developed into mini-research projects for courses in ecology, botany or genetics. This experiment has been performed by our undergraduates, and much en- thusiasm...
The American Biology Teacher. 1995; 574225–227 doi: https://doi.org/10.2307/4449975
Published: 01 April 1995
... lux R gene. Sal I, Bam H I and Pvu II are restriction endonuclease sites. James Slock is Professor of Biology at King's College, 133 N. River St., Wilkes- Barre, PA 18711. BIOLUMINESCENCE GENES 225 good source of information regarding molecular biology experiments, in- cluding plasmid mini...