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e-coli

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Journal Articles
The American Biology Teacher. 2005; 67287–92 doi: https://doi.org/10.2307/4451792
Published: 01 February 2005
... Biology Teacher 2001 Use of Long-Term E. coli Cultures To Study Generation of Genetic Diversity & Teach General Microbiology Laboratory Skills ANGELA PETRIE STEVEN E. FINKEL JARROD ERBE ntroductory microbiology students are expect- ed to learn a wide variety of laboratory techniques. These include...
Journal Articles
The American Biology Teacher. 2003; 65156–61 doi: https://doi.org/10.2307/4451434
Published: 01 January 2003
... 1993 Stix, G. (1998). The E. coli are coming. Scientific American, 279, 29. Stix 29 279 Scientific American 1998 Travis, J. (2000). Popularity of germ fighter raises concern. Science News, 157, 342. Travis 342 157 Science News 2000 EVOLUTION IN THE LAB: Biocide...
Journal Articles
The American Biology Teacher. 1994; 567424–428 doi: https://doi.org/10.2307/4449874
Published: 01 October 1994
... 1990 Isolation & Characterization of Plasmid from E. Coli Karl Schmidt Lawrence C. Davis Chemistry with kits is like cakes from a mix: You may get a satisfactory product, but you don't learn anything about the essential ingredients or pro- cesses involved. This is one of the gravest weaknesses of...
Journal Articles
The American Biology Teacher. 1985; 476362–364 doi: https://doi.org/10.2307/4448085
Published: 01 September 1985
... Enzymology 1963 A Method for Isolating DNA from E. Coli Richard Myers - Nucleic acids with attacnecl air bubbles can easily be "spooled" onto nearby glass rod. (Photo by Stafford Squier) The development of recombinant DNA techniques is an important area of study for inclusion in the high school biology...
Images
(A) Growth curve of  <b>E</b>. <b>coli</b>  grown at 25°C and 37°C. (B) Exponential phase...
Published: 01 May 2015
Figure 2. (A) Growth curve of E. coli grown at 25°C and 37°C. (B) Exponential phase of growth for E. coli at 25°C and 37°C. Figure 2. (A) Growth curve of E. coli grown at 25°C and 37°C. (B) Exponential phase of growth for E. coli at 25°C and 37°C. Figure 2. (A) Growth curve of E. coli grown at 25°C and 37°C. (B) Exponential phase of growth for E. coli at 25°C and 37°C. Figure 2. (A) Growth curve of E. coli grown at 25°C and 37°C. (B) Exponential phase of growth for E. coli at 25°C and 37°C. More
Images
Sample plate results. Purple colonies (dark circles) indicate  <b>E</b>. <b>coli</b> . (I...
Published: 01 September 2012
Figure 3. Sample plate results. Purple colonies (dark circles) indicate E. coli . (Imaging performed by Robert Fitton.) Figure 3. Sample plate results. Purple colonies (dark circles) indicate E. coli. (Imaging performed by Robert Fitton.) Figure 3. Sample plate results. Purple colonies (dark circles) indicate E. coli . (Imaging performed by Robert Fitton.) Figure 3. Sample plate results. Purple colonies (dark circles) indicate E. coli. (Imaging performed by Robert Fitton.) More
Journal Articles
The American Biology Teacher. 2018; 804305–307 doi: https://doi.org/10.1525/abt.2018.80.4.305
Published: 01 April 2018
... introduces students to a multidisciplinary approach to STEM and provides an opportunity to explore the use of science in different fields such as design, art, and industry. In this protocol extension students create living works of art on agar plates by “painting” with E. coli that express fluorescent...
Journal Articles
The American Biology Teacher. 2018; 803214–220 doi: https://doi.org/10.1525/abt.2018.80.3.214
Published: 01 March 2018
... resistance. Using an antibiotic disk diffusion assay, students compare the antibiotic resistance level of a harmless E. coli strain of bacteria over time. Then, students compare their lab data to the models, which together illustrate the roles that initial genetic variation and random mutation play in the...
Journal Articles
The American Biology Teacher. 2016; 789739–745 doi: https://doi.org/10.1525/abt.2016.78.9.739
Published: 01 November 2016
... activities utilizing the real-world bioscience behind wastewater treatment. The activities begin with a tour of a wastewater treatment facility, followed by a guided inquiry activity in which students enumerate E. coli levels from wastewater samples collected from different steps of the treatment process...
Journal Articles
The American Biology Teacher. 2015; 777526–531 doi: https://doi.org/10.1525/abt.2015.77.7.7
Published: 01 September 2015
..., the cloning of that gene into an expression vector, the amplification of this plasmid in E. coli, and the expression of fluorescent tubulin in yeast. Controls and validation steps are embedded throughout the project, as they would be in a genuine research project. This laboratory course more closely...
Journal Articles
The American Biology Teacher. 2020; 825315–322 doi: https://doi.org/10.1525/abt.2020.82.5.315
Published: 01 May 2020
... the plasmid found within the GFP(+) E. coli used in their experiment. The complete plasmid is 2000 base pairs (bp). Notice that the plasmid contains a Sal1 restriction-enzyme cutting site adjacent to the GFP gene. This short sequence could be cut using the Sal1 restriction enzyme, just as...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2020; 825279–288 doi: https://doi.org/10.1525/abt.2020.82.5.279
Published: 01 May 2020
... Escherichia coli . While sequencing part of the E. coli chromosome, he discovered several short repetitive sequences separated by short random spacer sequences. These short repeat sequences are referred to as CRISPR (clustered regularly interspaced short palindromic repeats). Ishino's paper famously...
Journal Articles
The American Biology Teacher. 2020; 824201–208 doi: https://doi.org/10.1525/abt.2020.82.4.201
Published: 01 April 2020
... their ability to produce antibacterial compounds that inhibit the growth of three types of bacteria ( Escherichia coli , Micrococcus luteus , and Mycobacterium smegmatis ; chosen to represent the Gram-negative, Gram-positive, and acid-fast cell wall, respectively). After incubation, the students...
Journal Articles
The American Biology Teacher. 2019; 819618–624 doi: https://doi.org/10.1525/abt.2019.81.9.618
Published: 01 December 2019
... chemistry 30–45 minutes Measurements of parameters including oxygen, temperature (air and water), pH, and electrical conductivity Bacterial 15–20 minutes Plating of collected water samples for detection of E. coli ; incubation period of 24 hours needed following plating Macroinvertebrates 45–75...
Journal Articles
The American Biology Teacher. 2019; 819680–685 doi: https://doi.org/10.1525/abt.2019.81.9.680
Published: 01 December 2019
...............................3.169-I Donnan equilibrium............................5.373-TT Drag............................2.115-I Drosophila ............................1.47-TT Drosophila melanogaster ............................4.269-I Drug target............................6.423-I E. Coli...
Journal Articles
The American Biology Teacher. 2019; 818577–581 doi: https://doi.org/10.1525/abt.2019.81.8.577
Published: 01 October 2019
... island causing the pathogenicity of the outbreak strain. A closer examination shows that the virulence factors responsible for the pathogenicity is a set of genes from Shigella transferred into E. coli . These videos prompt the inquiry of how scientists compare genomes. We then proceed with a brief...
Journal Articles
The American Biology Teacher. 2019; 816412–415 doi: https://doi.org/10.1525/abt.2019.81.6.412
Published: 01 August 2019
... vision(s) for interdisciplinary social studies . Social Studies Research and Practice , 11 , 177 – 189 . Idexx Laboratories (n.d.). Coliform/E. coli results in 24 hours . https://www.idexx.com/ . Wahrman, M.Z. ( 2016 ). Touch at your own risk . In The Hand Book: Surviving in a Germ...
Journal Articles
The American Biology Teacher. 2019; 817467–473 doi: https://doi.org/10.1525/abt.2019.81.7.467
Published: 01 September 2019
...); ( D ) comparison of the sterilization effect of different brands of hand soaps at different concentrations (2011, School of Materials Science); ( E ) “Evolution of Life” (2017, School of Biotechnology); ( F ) “Protein Synthesis” (2017, School of Biotechnology); ( G ) “Chinese Pavilion, Shanghai Expo...
Journal Articles
The American Biology Teacher. 2019; 81152–55 doi: https://doi.org/10.1525/abt.2019.81.1.52
Published: 01 January 2019
..., E. ( 2010 ). Plasmid DNA transformation of Escherichia coli: effect of heat shock temperature, duration, and cold incubation of CaCl 2 treated cells . International Journal of Biotechnology and Biochemistry , 6 , 561 – 568 . Wright, L.K. , & Newman, D.L. ( 2013 ). Using PCR to...
Images
Schematic of the steps involved in cloning and over-expression of carbonic ...
Published: 01 January 2018
plasmid containing the CA gene. (D) The recombinant plasmid was subsequently transformed into commercially available, over-expression strains such as E. coli BL21(DE3). (E) Over-expression of CA was induced with 0.5 IPTG. Figure 1. Schematic of the steps involved in cloning and over-expression of plasmid containing the CA gene. (D) The recombinant plasmid was subsequently transformed into commercially available, over-expression strains such as E. coli BL21(DE3). (E) Over-expression of CA was induced with 0.5 IPTG. Figure 1. Schematic of the steps involved in cloning and over-expression of More