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dna-primers

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Components of the set: (1) double-stranded <b>DNA</b> template; (2) two RNA <b>primer</b>...
Published: 01 January 2020
Figure 1. Components of the set: (1) double-stranded DNA template; (2) two RNA primers for replication; (3) three RNA primers for transcription; (4) seven pairs of primers for PCR; (5) free dNTPs in a bottle; (6) free NTPs in a bottle; (7) Petri dish; (8) rubber “stoppers”; (9) manual for Figure 1. Components of the set: (1) double-stranded DNA template; (2) two RNA primers for replication; (3) three RNA primers for transcription; (4) seven pairs of primers for PCR; (5) free dNTPs in a bottle; (6) free NTPs in a bottle; (7) Petri dish; (8) rubber “stoppers”; (9) manual for More
Journal Articles
The American Biology Teacher. 2013; 759682–691 doi: https://doi.org/10.1525/abt.2013.75.9.10
Published: 01 November 2013
... straightforward. This exercise is designed for use in high school environments as a stand-alone paper lab and as a primer to an actual wet-lab experience, if available. Upon conclusion of the experience, students will have confidence in understanding how DNA fingerprinting can constitute strong evidence in...
Journal Articles
The American Biology Teacher. 2012; 744256–260 doi: https://doi.org/10.1525/abt.2012.74.4.9
Published: 01 April 2012
.... In this molecular biology laboratory, students learn the steps of PCR with an emphasis on primer composition and annealing temperature, which they manipulate to test the effect on successful DNA amplification. Students design experiments to test their hypotheses, promoting a discovery-based approach...
Journal Articles
The American Biology Teacher. 2020; 823170–175 doi: https://doi.org/10.1525/abt.2020.82.3.170
Published: 01 March 2020
... Wizard Genomic DNA Purification Kit (Promega, Madison, WI). Next, they amplified the 16S gene from their DNA extractions using the primers 16Sar and 16Sbr ( Ivanova et al., 2007 ). Each reaction mix was made using a GoTaq Flexi DNA Polymerase Kit (Promega) and consisted of the following reagents (and...
Journal Articles
The American Biology Teacher. 2020; 82149–51 doi: https://doi.org/10.1525/abt.2020.82.1.49
Published: 01 January 2020
...Figure 1. Components of the set: (1) double-stranded DNA template; (2) two RNA primers for replication; (3) three RNA primers for transcription; (4) seven pairs of primers for PCR; (5) free dNTPs in a bottle; (6) free NTPs in a bottle; (7) Petri dish; (8) rubber “stoppers”; (9) manual for...
Journal Articles
The American Biology Teacher. 2019; 817492–501 doi: https://doi.org/10.1525/abt.2019.81.7.492
Published: 01 September 2019
... of the ITS2 region of the ribosomal gene ( Greene et al., 2000 ; Lauer et al., 2012 ). The Green GoTaq Mastermix is used to prepare 25 μL PCRs following recommendations of aliquots of DNA, primer, and sterile PCR water in the manufacturer's protocol. The success of the multiplex PCRs can be...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2019; 813162–167 doi: https://doi.org/10.1525/abt.2019.81.3.162
Published: 01 March 2019
... ). Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species . Frontiers in Zoology , 10 , 45 . Folmer, O. , Black, M. , Hoeh, W. , Lutz, R. & Vrijenhoek, R. ( 1994 ). DNA primers for amplification of mitochondrial cytochrome c...
Journal Articles
The American Biology Teacher. 2019; 813169–174 doi: https://doi.org/10.1525/abt.2019.81.3.169
Published: 01 March 2019
... Society B , 270 , S1, S96 – S99 . Ivanova, N.V. , Zemlak, T.S. , Hanner, R.H. & Hebert, P.D. ( 2007 ). Universal primer cocktails for fish DNA barcoding . Molecular Ecology Resources , 7 , 544 – 548 . Jo, H. , Ventura, M. , Vidal, N. , Gim, J.S. , Buchaca, T. , Barmuta, L.A...
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Sample data from student projects. Students are able to obtain isolated col...
Published: 01 March 2017
) and micrographs (B) from their enrichments (C). They monitor the growth of these enrichments by measuring optical density (turbidity) (D). They are also able to extract DNA from soils (E) and carry out PCR and gel electrophoresis; amplicons shown were generated using universal nifH primers (F). At the ) and micrographs (B) from their enrichments (C). They monitor the growth of these enrichments by measuring optical density (turbidity) (D). They are also able to extract DNA from soils (E) and carry out PCR and gel electrophoresis; amplicons shown were generated using universal nifH primers (F). At the More
Journal Articles
The American Biology Teacher. 2018; 804285–289 doi: https://doi.org/10.1525/abt.2018.80.4.285
Published: 01 April 2018
... were graduate students using this procedure for their thesis research (see Online Supplementary Material). Once the students isolate the DNA, they amplify sections of DNA specific to our target organism using the polymerase chain reaction (PCR) with primers and protocols outlined in Ficetola et al...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2018; 803198–202 doi: https://doi.org/10.1525/abt.2018.80.3.198
Published: 01 March 2018
... many teaching laboratories.) Grow isolated colonies in 100 mL Brain Heart Infusion Broth (Catalogue #C5121, Hardy Diagnostics, Santa Maria, CA) using either the Oxo or Anchor Hocking chamber as described above. Isolate genomic DNA from each isolated bacterium using the Wizard Genomic DNA...
Journal Articles
The American Biology Teacher. 2017; 799763–768 doi: https://doi.org/10.1525/abt.2017.79.9.763
Published: 01 November 2017
... Protocols — #1 #2 #3, 4, 5 #6 Reagents and expenses — DNeasy Blood and Tissue Kit ($3/sample) PCR primers ($15 total) HotStarTaq Master Mix ($2/sample) MinElute PCR Purification ($2.50/reaction) DNA seq. ($3–5/reaction) — Equipment — Dissecting tools Balance Vortexer Microcentrifuge...
Journal Articles
The American Biology Teacher. 2018; 80129–34 doi: https://doi.org/10.1525/abt.2018.80.1.29
Published: 01 January 2018
... analysis of psychrophilic carbonic anhydrases from P. profundum . PCR amplification of ɑ–CA and β–CA genes was carried out using P. profundum genomic DNA as a template. Gene specific primers were designed previously by the research fellow and synthesized by Eurofins MWG operon. The gene encoding for...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2017; 799702–710 doi: https://doi.org/10.1525/abt.2017.79.9.702
Published: 01 November 2017
... mimicking DNA replication in a controlled setting. The DNA to be copied is combined with a mixture of nucleotide bases, small sequences of DNA called primers, and a special version of DNA polymerase called Taq polymerase. This mixture is then placed in an instrument called a thermocycler, which as the...
Journal Articles
The American Biology Teacher. 2017; 798615–620 doi: https://doi.org/10.1525/abt.2017.79.8.615
Published: 01 October 2017
... Cold Spring Harbor Laboratory also contributed to the early work on telomeres. Since DNA replication can proceed only in the 5′ to 3′ direction and requires RNA primers that are subsequently hydrolyzed, gaps are formed ( Figure 1 ). These gaps are filled using the adjacent Okazaki fragments as primers...
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Sample whiteboard for instruction and discussion of the M&amp;M exercise. (...
Published: 01 August 2016
Figure 1. Sample whiteboard for instruction and discussion of the M&amp;M exercise. ( A ) Visual representation of the non-template (right) and template strands (left) based on the worksheet accompanying the M&amp;M exercise ( Appendix ). The primer from the worksheet is drawn next to the DNA Figure 1. Sample whiteboard for instruction and discussion of the M&amp;M exercise. ( A ) Visual representation of the non-template (right) and template strands (left) based on the worksheet accompanying the M&amp;M exercise ( Appendix ). The primer from the worksheet is drawn next to the DNA More
Images
Partial hellbender single-strand <b>DNA</b> sequence with microsatellite (dark gra...
Published: 01 May 2014
Figure 4. Partial hellbender single-strand DNA sequence with microsatellite (dark gray) and primer (light gray) sequences highlighted ( Chudyk, 2013 ). Figure 4. Partial hellbender single-strand DNA sequence with microsatellite (dark gray) and primer (light gray) sequences highlighted (Chudyk Figure 4. Partial hellbender single-strand DNA sequence with microsatellite (dark gray) and primer (light gray) sequences highlighted ( Chudyk, 2013 ). Figure 4. Partial hellbender single-strand DNA sequence with microsatellite (dark gray) and primer (light gray) sequences highlighted (Chudyk More
Journal Articles
The American Biology Teacher. 2017; 793191–197 doi: https://doi.org/10.1525/abt.2017.79.3.191
Published: 01 March 2017
...) and micrographs (B) from their enrichments (C). They monitor the growth of these enrichments by measuring optical density (turbidity) (D). They are also able to extract DNA from soils (E) and carry out PCR and gel electrophoresis; amplicons shown were generated using universal nifH primers (F). At the...
Journal Articles
The American Biology Teacher. 2017; 793208–223 doi: https://doi.org/10.1525/abt.2017.79.3.208
Published: 01 March 2017
..., morphological or antibody staining, DNA and RNA purification, protein extraction, and isolation and culture of bacterial strains. The goals of these activities are to teach: (1) how to maintain a model system, with a rigorous and consistent protocol that guarantees experimental reproducibility; (2) how to...
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<b>Primer</b> design is critical to this exercise. Each set of chromosomes will re...
Published: 01 November 2013
Figure 1. Primer design is critical to this exercise. Each set of chromosomes will require a set of two primers that bookend the VNTRs as shown here. One primer must anneal to the top strand of DNA and allow for the generation of a new bottom strand toward the VNTR. The other primer must anneal Figure 1. Primer design is critical to this exercise. Each set of chromosomes will require a set of two primers that bookend the VNTRs as shown here. One primer must anneal to the top strand of DNA and allow for the generation of a new bottom strand toward the VNTR. The other primer must anneal More