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dna-extraction

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Journal Articles
The American Biology Teacher. 2020; 822132 doi: https://doi.org/10.1525/abt.2020.82.2.132
Published: 01 February 2020
...Jeffrey D. Sack Learn.Genetics: DNA Extraction ( Genetic Science Learning Center , https://learngendev.azurewebsites.net/content/labs/extraction/ ) © 2020 National Association of Biology Teachers. All rights reserved. Please direct all requests for permission to photocopy or reproduce...
Journal Articles
The American Biology Teacher. 2020; 823170–175 doi: https://doi.org/10.1525/abt.2020.82.3.170
Published: 01 March 2020
... research project in which students worked in groups to investigate the prevalence of fish mislabeling in local restaurants and grocery stores using DNA barcoding. During the experimental process, students learned fundamental molecular techniques like DNA extraction, polymerase chain reaction, gel...
Journal Articles
The American Biology Teacher. 2016; 787582–590 doi: https://doi.org/10.1525/abt.2016.78.7.582
Published: 01 September 2016
...Kadir Demir This transformed DNA-extraction lab activity offers a framework that strategically draws upon the essential elements of both scientific and effective teaching practices to establish an alternative approach to the teaching and learning of science. The pedagogical methods utilized...
Journal Articles
The American Biology Teacher. 2012; 747485–489 doi: https://doi.org/10.1525/abt.2012.74.7.10
Published: 01 September 2012
... collaborated with teachers in professional development workshops to transform “cookbook” activities into engaging laboratory experiences. We show how to change the common classroom activity of DNA extraction of wheat germ to integrate cooperative group strategies, graphic organizers, and classroom discussions...
Images
Preparation of  Lentinula edodes  for <b>DNA</b> <b>extraction</b>. (A) A shiitake mushro...
Published: 01 February 2016
Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom broken in half. (B) The mushroom was shredded using scissors. (C) The shredded mushroom was put into a mortar and ground. Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom broken in half. (B) The mushroom was shredded using scissors. (C) The shredded mushroom was put into a mortar and ground. Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom More
Images
Preparation of  Musa acuminata  for <b>DNA</b> <b>extraction</b>. (A) About 10 g of banan...
Published: 01 February 2016
Figure 4. Preparation of Musa acuminata for DNA extraction. (A) About 10 g of banana fruit. (B) The banana was crushed using a spatula. (C) Crushed banana. Figure 4. Preparation of Musa acuminata for DNA extraction. (A) About 10 g of banana fruit. (B) The banana was crushed using a spatula Figure 4. Preparation of Musa acuminata for DNA extraction. (A) About 10 g of banana fruit. (B) The banana was crushed using a spatula. (C) Crushed banana. Figure 4. Preparation of Musa acuminata for DNA extraction. (A) About 10 g of banana fruit. (B) The banana was crushed using a spatula More
Images
Preparation of  Undaria pinnatifida  for <b>DNA</b> <b>extraction</b>. (A) Commercially a...
Published: 01 February 2016
Figure 2. Preparation of Undaria pinnatifida for DNA extraction. (A) Commercially available dried brown seaweed was immersed in water. (B) Wet seaweed after immersion. (C) Water was removed by pressing the wet seaweed against a paper towel. (D) The seaweed was shredded using scissors. (E) The Figure 2. Preparation of Undaria pinnatifida for DNA extraction. (A) Commercially available dried brown seaweed was immersed in water. (B) Wet seaweed after immersion. (C) Water was removed by pressing the wet seaweed against a paper towel. (D) The seaweed was shredded using scissors. (E) The More
Images
Preparation of liver from  Gallus gallus domestica  for <b>DNA</b> <b>extraction</b>. (A)...
Published: 01 February 2016
Figure 5. Preparation of liver from Gallus gallus domestica for DNA extraction. (A) Frozen liver was put into a glass beaker, which was immersed in a vessel containing hot water for 1 minute to thaw the liver. (B) Thawed liver was cut to appropriate size using a kitchen knife. (C) Cut liver was Figure 5. Preparation of liver from Gallus gallus domestica for DNA extraction. (A) Frozen liver was put into a glass beaker, which was immersed in a vessel containing hot water for 1 minute to thaw the liver. (B) Thawed liver was cut to appropriate size using a kitchen knife. (C) Cut liver was More
Images
(Left) An attempt to organize the steps of <b>DNA</b> <b>extraction</b> of wheat germ. (R...
Published: 01 September 2012
Figure 1. (Left) An attempt to organize the steps of DNA extraction of wheat germ. (Right) The correct steps. Figure 1. (Left) An attempt to organize the steps of DNA extraction of wheat germ. (Right) The correct steps. Figure 1. (Left) An attempt to organize the steps of DNA extraction of wheat germ. (Right) The correct steps. Figure 1. (Left) An attempt to organize the steps of DNA extraction of wheat germ. (Right) The correct steps. More
Journal Articles
The American Biology Teacher. 2016; 782118–126 doi: https://doi.org/10.1525/abt.2016.78.2.118
Published: 01 February 2016
...Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom broken in half. (B) The mushroom was shredded using scissors. (C) The shredded mushroom was put into a mortar and ground. Figure 3. Preparation of Lentinula edodes for DNA extraction. (A) A shiitake mushroom...
Journal Articles
The American Biology Teacher. 2019; 813162–167 doi: https://doi.org/10.1525/abt.2019.81.3.162
Published: 01 March 2019
... attempt to integrate ecological or environmental analysis with genetic analysis and DNA extraction techniques, making this lab series worth exploring at the undergraduate level. References Carew, M.E. , Pettigrove, V.J. , Metzeling, L. & Hoffmann, A.A. ( 2013...
Journal Articles
The American Biology Teacher. 2019; 817492–501 doi: https://doi.org/10.1525/abt.2019.81.7.492
Published: 01 September 2019
... polymerase chain reaction to detect Coccidioides spp. in DNA extracts from soil samples collected by students across different soil environments. Additionally, this exercise will teach students how to use publicly available data to investigate disease incidence over time and characterize soils the pathogen...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 1998; 604276–280 doi: https://doi.org/10.2307/4450471
Published: 01 April 1998
... Mullis, K.B. & Faloona, F.A. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reac- tion. Methods Enzymology, 155, 335- 351. Mullis 335 155 Methods Enzymology 1987 Ogram, A., Sayler, G.S. & Barkay, T. (1987). The extraction and purifica- tion of microbial...
Journal Articles
The American Biology Teacher. 1993; 552100–101 doi: https://doi.org/10.2307/4449594
Published: 01 February 1993
... Sourcebook of Biotechnology Activ- ities. Reston, VA: NABT & Research Tri- angle Park, NC: North Carolina Biotech- nology Center. Rasmussen A Sourcebook of Biotechnology Activities 1990 How-To-Do-It Microscopic Titration & Extraction of DNA from Liver Lois T. Mayo Robert D. Curtright George E...
Journal Articles
The American Biology Teacher. 1968; 309755–756 doi: https://doi.org/10.2307/4442273
Published: 01 November 1968
.... Experimental biochemistry, 1964 WV. H. Freeman and Co. San Francisco, California. Clark Experimental biochemistry 1964 Extraction of DNA * Louis Palmeri, Bethpage High School, Bethpage, New York A laboratory exercise for advanced placement students that usually produces satisfactory results and...
Journal Articles
The American Biology Teacher. 2017; 799702–710 doi: https://doi.org/10.1525/abt.2017.79.9.702
Published: 01 November 2017
... biology course or a forensic science course. During this lesson, students examine a crime scene diagram and discuss evidence collection. Students then conduct a hands-on activity extracting DNA from wheat germ to simulate how DNA would be isolated from crime scene samples. Lastly the students will analyze...
Journal Articles
The American Biology Teacher. 2018; 804285–289 doi: https://doi.org/10.1525/abt.2018.80.4.285
Published: 01 April 2018
... curriculum, we developed an exercise using environmental DNA (eDNA) analysis. eDNA sampling involves extracting and amplifying the DNA from specific organisms from an environmental sample, rather than from the organisms themselves, and has been rapidly adopted by conservation practitioners around the world...
Includes: Supplementary data
Images
Beakers containing <b>extracted</b> <b>DNA</b>, placed on the diagram of the five-kingdom...
Published: 01 February 2016
Figure 13. Beakers containing extracted DNA, placed on the diagram of the five-kingdom phylogenetic tree in the last procedure to connect unity and diversity. Figure 13. Beakers containing extracted DNA, placed on the diagram of the five-kingdom phylogenetic tree in the last procedure to Figure 13. Beakers containing extracted DNA, placed on the diagram of the five-kingdom phylogenetic tree in the last procedure to connect unity and diversity. Figure 13. Beakers containing extracted DNA, placed on the diagram of the five-kingdom phylogenetic tree in the last procedure to More
Journal Articles
The American Biology Teacher. 2017; 793191–197 doi: https://doi.org/10.1525/abt.2017.79.3.191
Published: 01 March 2017
... sample. Students work in a research laboratory making nitrogen-free media; culturing, isolating, and identifying microorganisms; extracting soil DNA; and amplifying the 16S rRNA and nifH genes. We administer a pre-test and a post-test, and students present their research both in a short talk and with a...
Journal Articles
The American Biology Teacher. 2020; 824234–240 doi: https://doi.org/10.1525/abt.2020.82.4.234
Published: 01 April 2020
... leaf litter samples, which were extracted and handed over to our campaign partner Barcoding Fauna Bavarica (part of the International Barcode of Life Initiative), which intends to establish a library of DNA barcodes of all Bavarian species. The advantage of this collaborative citizen science project...