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diagnostic-pcr

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Journal Articles
The American Biology Teacher. 2014; 764254–258 doi: https://doi.org/10.1525/abt.2014.76.4.7
Published: 01 April 2014
...Sharon Gusky In this activity, freshman college students learn biotechnology techniques while playing the role of a laboratory technician. They perform simulations of three diagnostic tests used to screen newborns for cystic fibrosis. By performing an ELISA, a PCR analysis, and a conductivity test...
Images
Flowchart showing the structure of the exercise, highlighting the different...
Published: 01 September 2019
gray boxes, left), detection of the pathogen in soil samples collected from diverse types of soils using diagnostic PCR (medium gray boxes, center), and working with disease incidence data (dark gray boxes, right) (investigation part). gray boxes, left), detection of the pathogen in soil samples collected from diverse types of soils using diagnostic PCR (medium gray boxes, center), and working with disease incidence data (dark gray boxes, right) (investigation part). More
Images
Soil sampling plan showing different soil types on the CSUB campus. The are...
Published: 01 September 2019
loam), 127 (Granoso sandy loam), 174 (Kimberlina fine sandy loam). Two lab sections participated in this exercise. Sites where the pathogen was detected via diagnostic PCR are indicated with black dots within white circles. Coccidioides immitis negative sites are denoted by white dots. Letters next loam), 127 (Granoso sandy loam), 174 (Kimberlina fine sandy loam). Two lab sections participated in this exercise. Sites where the pathogen was detected via diagnostic PCR are indicated with black dots within white circles. Coccidioides immitis negative sites are denoted by white dots. Letters next More
Journal Articles
The American Biology Teacher. 2019; 817492–501 doi: https://doi.org/10.1525/abt.2019.81.7.492
Published: 01 September 2019
... gray boxes, left), detection of the pathogen in soil samples collected from diverse types of soils using diagnostic PCR (medium gray boxes, center), and working with disease incidence data (dark gray boxes, right) (investigation part). ...
Includes: Supplementary data
Journal Articles
The American Biology Teacher. 2019; 813169–174 doi: https://doi.org/10.1525/abt.2019.81.3.169
Published: 01 March 2019
..., including describing the requirements and results of PCR Describe the process and application of agarose gel electrophoresis Construct a hypothetical food web using student-generated prey data Describe potential ecological and economic impacts of invasive fish species Perform a fish...
Journal Articles
The American Biology Teacher. 2018; 803198–202 doi: https://doi.org/10.1525/abt.2018.80.3.198
Published: 01 March 2018
... genomic DNA-polymerase chain reaction (PCR) master mix according to the manufacturer's instructions (GeneMateTaq Master Mix 2X, Bioexpress-VWR, Batavia, IL). Perform a PCR protocol using the Techne TC412 (Keison Products, Grants Pass, OR), or similar, thermocycler and the following paramaters: 94°C...
Journal Articles
The American Biology Teacher. 2017; 794301–304 doi: https://doi.org/10.1525/abt.2017.79.4.301
Published: 01 April 2017
... multiplex PCR-ELISA method for the genetic authentication of Thunnus species and Katsuwonus pelamis in food products . Food Chemistry , 180 , 9 – 16 . Tsujii, M. , Kawano, S. , Tsuji, S. , Sawaoka, H. , Hori, M. , & DuBois, R. N. ( 1998 ). Cyclooxegenase regulates angiogenesis induced by...
Journal Articles
The American Biology Teacher. 2016; 786516–522 doi: https://doi.org/10.1525/abt.2016.78.6.516
Published: 01 August 2016
... & M's. See Figure 2 for a sample outcome showing the template strand. Given this PCR primer, represent the set of different DNA fragment(s) that you could synthesize if a ddNTP (Peanut M & M) was incorporated into your products at low frequency. Photograph your set of products. See...
Journal Articles
The American Biology Teacher. 2016; 783190–197 doi: https://doi.org/10.1525/abt.2016.78.3.190
Published: 01 March 2016
... disease (EVD) epidemic module. Topic Area of Lesson Extension Description Source Biotechnology Additional information can be provided on assays such as ELISA and polymerase chain reaction (PCR), which are often used to diagnose infectious diseases. A stand-alone lesson on EVD takes...
Journal Articles
The American Biology Teacher. 2016; 7817–13 doi: https://doi.org/10.1525/abt.2016.78.1.7
Published: 01 January 2016
... (PCR) used in research, forensics, and medical diagnostics. Among the most easily handled extremophiles in the lab are the Haloarchaea, which are being used for studies as diverse as medical research and astrobiology (S. DasSarma, 2006 , 2007 ; P. DasSarma et al., 2010a ). An ideal second hands...
Images
Molecular <b>diagnostics</b>. Overview of the MGDP <b>diagnostic</b> laboratory exercise....
Published: 01 April 2012
Figure 1. Molecular diagnostics. Overview of the MGDP diagnostic laboratory exercise. (A) Map of the mitochondrial genome, indicating the location of the L1 (tRNA Leu ) gene and approximate site of faux PCR amplification. (B) Restriction mapping (“Rre I”) strategy for identification of A3243G Figure 1. Molecular diagnostics. Overview of the MGDP diagnostic laboratory exercise. (A) Map of the mitochondrial genome, indicating the location of the L1 (tRNA Leu ) gene and approximate site of faux PCR amplification. (B) Restriction mapping (“Rre I”) strategy for identification of A3243G More
Journal Articles
The American Biology Teacher. 2012; 744256–260 doi: https://doi.org/10.1525/abt.2012.74.4.9
Published: 01 April 2012
... teaching laboratory: identification of bacterial unknowns. Biochemistry and Molecular Biology Education , 30 , 394–397. Baker, W.P., Jarman, M., Ronstadt-Moore, C. & Rhodes, S. (1999). Introducing undergraduates to diagnostic virology using PCR. Journal of College Science Teaching , 28 , 423–426...
Journal Articles
The American Biology Teacher. 2013; 75129–33 doi: https://doi.org/10.1525/abt.2013.75.1.7
Published: 01 January 2013
... of MBt is for students to use virtual laboratory experiences to provide key diagnostic insight into which viruses are causing each of four levels’ epidemiological outbreak. The game introduces biotechnology protocols (such as DNA extraction, PCR, and reverse transcription) not just as content to be...
Journal Articles
The American Biology Teacher. 2012; 742128 doi: https://doi.org/10.1525/abt.2012.74.2.14
Published: 01 February 2012
... reactions. One reaction uses primers for tubulin, a gene expressed in all plants, to provide evidence of amplifiable DNA in the extraction. The second primer set amplifies the 35S promoter, a common promoter used in plants to drive expression of a transgene. The presence of a 35S PCR product is diagnostic...
Journal Articles
The American Biology Teacher. 2012; 749667–671 doi: https://doi.org/10.1525/abt.2012.74.9.14
Published: 01 November 2012
... Phylogenetics.......................4:244-I, 5:332-H Phytophthora ramorum .......................3:191-Q Plant–animal interactions.......................7:509-I Pollen germination.......................8:575-H Pollution.......................7:479-I Polymerase chain reaction (PCR...
Journal Articles
The American Biology Teacher. 2012; 744224–230 doi: https://doi.org/10.1525/abt.2012.74.4.4
Published: 01 April 2012
...Figure 1. Molecular diagnostics. Overview of the MGDP diagnostic laboratory exercise. (A) Map of the mitochondrial genome, indicating the location of the L1 (tRNA Leu ) gene and approximate site of faux PCR amplification. (B) Restriction mapping (“Rre I”) strategy for identification of A3243G...
Journal Articles
The American Biology Teacher. 2011; 736331–335 doi: https://doi.org/10.1525/abt.2011.73.6.5
Published: 01 August 2011
... clinical/diagnostic and basic microbiology research worlds, identification of organisms is often achieved by employing molecular biology techniques, including the polymerase chain reaction (PCR) and DNA sequencing. ...
Journal Articles
The American Biology Teacher. 2007; 695280–286 doi: https://doi.org/10.2307/4452157
Published: 01 May 2007
... developed by the FBI is now a PCR-based, fluorescently-labeled amplification of microsatellite mark- ers, followed by capillary electrophoresis. Although this technique requires specialized instrumentation and expen- sive reagents, it is an excellent method to teach students Mendelian genetics and genome...
Journal Articles
The American Biology Teacher. 2004; 663222–223 doi: https://doi.org/10.2307/4451657
Published: 01 March 2004
... methods to identify the exact genus and species of bacteria. There are six parts to the laboratory experi- ment that the student must com- plete. Part One involves prepara- tion of the sample for use in PCR (Polymerase Chain Reaction). The Vnote window gives a nice description of what chemicals are being...
Journal Articles
The American Biology Teacher. 2002; 643212–218 doi: https://doi.org/10.2307/4451279
Published: 01 March 2002
..., Southwestern Hispanics, and Orientals. Journal of Forensic Science, 40, 38-44. Budowle 38 40 Journal of Forensic Science 1995 Budowle, B., Chakraborty, R., Giusti, A.M., Eisenberg, Aj. & Allen, R.C. (1991). Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. American...