Project-based learning and action research are powerful pedagogies in improving science education. We implemented a semester-long course using project-based action research to help students apply biotechnology knowledge learned in the classroom to the real world. Students had several choices to make in the project: working individually or as a team, selecting a topic of interest, and targeting a local community group. To enhance teachers’ abilities to lead students through action projects, we describe the framework, provide class data, and discuss benefits and challenges encountered. This course could serve as a model of how project-based action research can benefit student learning in biotechnology.