Idiograms are graphical representations in which chromosomes appear ordered by their relative size, homology groups, and the presence of cytogenetic landmarks such as centromeres, secondary constrictions, and banding patterns. They have become crucial elements in cytogenetic research. Here, we describe a simple protocol for spreadsheet software to draw idiograms and map any type of sequence onto them. These graphs can be easily used to present research results and can become the basis to raise and resolve questions related to different biological issues. The methodology is inexpensive, easy to follow, and allows customization to meet a wide range of educational scenarios focused in genetics and evolution.

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